Advanced

In vivo gene delivery to proliferating cells in the striatum generated in response to a 6-hydroxydopamine lesion of the nigro-striatal dopamine pathway

Morris-Irvin, Dwain LU ; Kirik, Deniz LU ; Björklund, Anders LU and Thompson, Lachlan LU (2008) In Neurobiology of Disease 30(3). p.343-352
Abstract
The degeneration of neurons in the mammalian brain is commonly associated with the division of cells located in the damaged area. The aim of the present study has been to characterise the phenotype of newly born cells in the striatum of adult rats following 6-hydroxydopamine lesion of the nigro-striatal pathway. Newborn cells were identified through labelling with either bromodeoxyuridine or retrovirus encoding green fluorescence protein. We report here that the overwhelming majority of these cells have glial characteristics. In order to promote the generation of new neurons we retrovirally introduced either the noggin or neurogenin2 genes into newborn cells following the 6-hydroxydopamine lesion. Transduction with neurogenin2 resulted in... (More)
The degeneration of neurons in the mammalian brain is commonly associated with the division of cells located in the damaged area. The aim of the present study has been to characterise the phenotype of newly born cells in the striatum of adult rats following 6-hydroxydopamine lesion of the nigro-striatal pathway. Newborn cells were identified through labelling with either bromodeoxyuridine or retrovirus encoding green fluorescence protein. We report here that the overwhelming majority of these cells have glial characteristics. In order to promote the generation of new neurons we retrovirally introduced either the noggin or neurogenin2 genes into newborn cells following the 6-hydroxydopamine lesion. Transduction with neurogenin2 resulted in the production of cells resembling neuroblasts, however these cells did not appear to survive. Noggin transduction did not result in the generation of new neurons, but interestingly, greatly increased the number of oligodendrocytes generated from newborn cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Parkinson's disease, neurogenin2, noggin, proliferation, retrovirus
in
Neurobiology of Disease
volume
30
issue
3
pages
343 - 352
publisher
Elsevier
external identifiers
  • wos:000256278600007
  • scopus:43649087693
ISSN
0969-9961
DOI
10.1016/j.nbd.2008.02.006
language
English
LU publication?
yes
id
45e42d20-83cf-437c-833c-c7f37c1b786b (old id 1201595)
date added to LUP
2008-09-15 11:30:00
date last changed
2017-01-01 04:28:28
@article{45e42d20-83cf-437c-833c-c7f37c1b786b,
  abstract     = {The degeneration of neurons in the mammalian brain is commonly associated with the division of cells located in the damaged area. The aim of the present study has been to characterise the phenotype of newly born cells in the striatum of adult rats following 6-hydroxydopamine lesion of the nigro-striatal pathway. Newborn cells were identified through labelling with either bromodeoxyuridine or retrovirus encoding green fluorescence protein. We report here that the overwhelming majority of these cells have glial characteristics. In order to promote the generation of new neurons we retrovirally introduced either the noggin or neurogenin2 genes into newborn cells following the 6-hydroxydopamine lesion. Transduction with neurogenin2 resulted in the production of cells resembling neuroblasts, however these cells did not appear to survive. Noggin transduction did not result in the generation of new neurons, but interestingly, greatly increased the number of oligodendrocytes generated from newborn cells.},
  author       = {Morris-Irvin, Dwain and Kirik, Deniz and Björklund, Anders and Thompson, Lachlan},
  issn         = {0969-9961},
  keyword      = {Parkinson's disease,neurogenin2,noggin,proliferation,retrovirus},
  language     = {eng},
  number       = {3},
  pages        = {343--352},
  publisher    = {Elsevier},
  series       = {Neurobiology of Disease},
  title        = {In vivo gene delivery to proliferating cells in the striatum generated in response to a 6-hydroxydopamine lesion of the nigro-striatal dopamine pathway},
  url          = {http://dx.doi.org/10.1016/j.nbd.2008.02.006},
  volume       = {30},
  year         = {2008},
}