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Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination

Polonis, Victoria R; Brown, Bruce K; Borges, Andrew Rosa; Zolla-Pazner, Susan; Dimitrov, Dimiter S; Zhang, Mei-Yun; Barnett, Susan W; Ruprecht, Ruth M; Scarlatti, Gabriella and Fenyö, Eva Maria LU , et al. (2008) In Virology 375(2). p.315-320
Abstract
In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of... (More)
In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved. (Less)
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Contribution to journal
publication status
published
subject
keywords
vaccine immune assessment, standardization, pseudovirus, assays, neutralization, human immunodeficiency virus type I (HIV-1), antibodies
in
Virology
volume
375
issue
2
pages
315 - 320
publisher
Elsevier
external identifiers
  • wos:000256106200001
  • scopus:43249120426
ISSN
1096-0341
DOI
10.1016/j.virol.2008.02.007
language
English
LU publication?
yes
id
736d286c-42d8-475c-a593-806e48b56e80 (old id 1201927)
date added to LUP
2008-09-15 13:00:15
date last changed
2017-08-27 04:33:41
@article{736d286c-42d8-475c-a593-806e48b56e80,
  abstract     = {In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.},
  author       = {Polonis, Victoria R and Brown, Bruce K and Borges, Andrew Rosa and Zolla-Pazner, Susan and Dimitrov, Dimiter S and Zhang, Mei-Yun and Barnett, Susan W and Ruprecht, Ruth M and Scarlatti, Gabriella and Fenyö, Eva Maria and Montefiori, David C and McCutchan, Francine E and Michael, Nelson L},
  issn         = {1096-0341},
  keyword      = {vaccine immune assessment,standardization,pseudovirus,assays,neutralization,human immunodeficiency virus type I (HIV-1),antibodies},
  language     = {eng},
  number       = {2},
  pages        = {315--320},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination},
  url          = {http://dx.doi.org/10.1016/j.virol.2008.02.007},
  volume       = {375},
  year         = {2008},
}