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Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants

Tikkanen, Mikko; Nurmi, Markus; Suorsa, Marjaana; Danielsson, Ravi LU ; Mamedov, Fikret; Styring, Stenbjoern and Aro, Eva-Mari (2008) In Biochimica et Biophysica Acta - Bioenergetics 1777(5). p.425-432
Abstract
Phosphorylation-dependent movement of the light-harvesting complex II (LHCII) between photosystern II (PSII) and photosystem I (PSI) takes place in order to balance the function of the two photosystems. Traditionally, the phosphorylatable fraction of LHCII has been considered as the functional unit of this dynamic regulation. Here, a mechanical fractionation of the thylakoid membrane of Spinacia oleracea was performed from leaves both in the phosphorylated state (low light, LL) and in the dephosphorylated state (dark, D) in order to compare the phosphorylation-dependent protein movements with the excitation changes occurring in the two photosystems upon LHCII phosphorylation, Despite the fact that several LHCII proteins migrate to stroma... (More)
Phosphorylation-dependent movement of the light-harvesting complex II (LHCII) between photosystern II (PSII) and photosystem I (PSI) takes place in order to balance the function of the two photosystems. Traditionally, the phosphorylatable fraction of LHCII has been considered as the functional unit of this dynamic regulation. Here, a mechanical fractionation of the thylakoid membrane of Spinacia oleracea was performed from leaves both in the phosphorylated state (low light, LL) and in the dephosphorylated state (dark, D) in order to compare the phosphorylation-dependent protein movements with the excitation changes occurring in the two photosystems upon LHCII phosphorylation, Despite the fact that several LHCII proteins migrate to stroma lamellae when LHCII is phosphorylated, no increase occurs in the 77 K fluorescence emitted from PSI in this membrane fraction. On the contrary, such an increase in fluorescence occurs in the grana margin fraction, and the functionally important mobile unit is the PSI-LHCI complex. A new model for LHCII phosphorylation driven regulation of relative PSII/PSI excitation thus emphasises an increase in PSI absorption cross-section occurring in grana margins upon LHCII phosphorylation and resulting from the movement of PSI-LHCI complexes from stroma lamellae and subsequent co-operation with the P-LHCII antenna from the grana. The grana margins probably give a flexibility for regulation of linear and cyclic electron flow in plant chloroplasts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
state transition, phosphorylation, thylakoid protein, photosynthesis, photosystem II, LHCII, photosystem I
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1777
issue
5
pages
425 - 432
publisher
Elsevier
external identifiers
  • wos:000256145000005
  • scopus:43049144541
ISSN
0005-2728
DOI
10.1016/j.bbabio.2008.02.001
language
English
LU publication?
yes
id
0e651973-961b-417e-93d0-e3b7ce281d78 (old id 1202171)
date added to LUP
2008-09-15 15:47:14
date last changed
2017-08-27 04:52:13
@article{0e651973-961b-417e-93d0-e3b7ce281d78,
  abstract     = {Phosphorylation-dependent movement of the light-harvesting complex II (LHCII) between photosystern II (PSII) and photosystem I (PSI) takes place in order to balance the function of the two photosystems. Traditionally, the phosphorylatable fraction of LHCII has been considered as the functional unit of this dynamic regulation. Here, a mechanical fractionation of the thylakoid membrane of Spinacia oleracea was performed from leaves both in the phosphorylated state (low light, LL) and in the dephosphorylated state (dark, D) in order to compare the phosphorylation-dependent protein movements with the excitation changes occurring in the two photosystems upon LHCII phosphorylation, Despite the fact that several LHCII proteins migrate to stroma lamellae when LHCII is phosphorylated, no increase occurs in the 77 K fluorescence emitted from PSI in this membrane fraction. On the contrary, such an increase in fluorescence occurs in the grana margin fraction, and the functionally important mobile unit is the PSI-LHCI complex. A new model for LHCII phosphorylation driven regulation of relative PSII/PSI excitation thus emphasises an increase in PSI absorption cross-section occurring in grana margins upon LHCII phosphorylation and resulting from the movement of PSI-LHCI complexes from stroma lamellae and subsequent co-operation with the P-LHCII antenna from the grana. The grana margins probably give a flexibility for regulation of linear and cyclic electron flow in plant chloroplasts.},
  author       = {Tikkanen, Mikko and Nurmi, Markus and Suorsa, Marjaana and Danielsson, Ravi and Mamedov, Fikret and Styring, Stenbjoern and Aro, Eva-Mari},
  issn         = {0005-2728},
  keyword      = {state transition,phosphorylation,thylakoid protein,photosynthesis,photosystem II,LHCII,photosystem I},
  language     = {eng},
  number       = {5},
  pages        = {425--432},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants},
  url          = {http://dx.doi.org/10.1016/j.bbabio.2008.02.001},
  volume       = {1777},
  year         = {2008},
}