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Tympanic membrane changes in experimental acute otitis media and myringotomy

Alzbutiene, Giedre; Hermansson, Ann LU ; Caye-Thomasen, Per and Kinduris, Vytenis (2008) In Medicina 44(4). p.313-321
Abstract
Objective. The present experimental study explored pathomorphological changes and calcium depositions in the tympanic membrane during experimental acute otitis media caused by nontypeable Haemophilus influenzae in myringotomized and nonmyringotomized ears. Material and methods. A rat model of experimental acute otitis media caused by nontypeable Haemophilus influenzae was employed Sixteen Sprague-Dawley rats were used. Four days following middle ear inoculation, a bilateral myringotomy was performed in six randomly selected animals. Another group of 10 animals was inoculated only. On days 4, 7, 14, and 28, after inoculation, two animals from each group were sacrificed The temporal bones were removed and the tympanic membranes were... (More)
Objective. The present experimental study explored pathomorphological changes and calcium depositions in the tympanic membrane during experimental acute otitis media caused by nontypeable Haemophilus influenzae in myringotomized and nonmyringotomized ears. Material and methods. A rat model of experimental acute otitis media caused by nontypeable Haemophilus influenzae was employed Sixteen Sprague-Dawley rats were used. Four days following middle ear inoculation, a bilateral myringotomy was performed in six randomly selected animals. Another group of 10 animals was inoculated only. On days 4, 7, 14, and 28, after inoculation, two animals from each group were sacrificed The temporal bones were removed and the tympanic membranes were dissected, followed by paraffin embedding. Adjacent sections were stained with PAS-alcian blue for basic histopathological observations and by von Kossa method for determination of calcium phosphate depositions. Results. Particularly intense invasion of polymorphonuclear neutrophil leukocytes was, seen on day 4 after inoculation. The highest infiltration of macrophages was observed on day 7. The peak number of lymphocytes was seen on day 14. No difference occurred in the number of polymorphonuclear leukocytes in myringotomized and nonmyringotomized tympanic membranes. The infiltration with lymphocytes and activated macrophages in all parts of the myringotomized tympanic membranes was statistically significantly higher than in the nonmyringotomized animals. The total amount of interstitial calcium phosphate depositions during days 7, 14, and 28 of study was statistically higher in the sections of pars tensa from myringotomized membranes compared to the nonmyringotomized membranes. Conclusion. Nontypeable Haemophilus influenzae-induced acute otitis media and myringotomy provoke more extensive inflammatory reaction with microcalcification in the tympanic membranes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
myringosclerosis, acute otitis media, myringotomy, animal model
in
Medicina
volume
44
issue
4
pages
313 - 321
publisher
Kauno medicinos universitetas
external identifiers
  • wos:000255998900009
  • scopus:44949117675
ISSN
1648-9144
language
English
LU publication?
yes
id
4403c4e0-08c4-44c7-856a-3152be74e9a0 (old id 1203321)
alternative location
http://medicina.kmu.lt/0804/0804-09e.pdf
date added to LUP
2008-09-15 16:32:22
date last changed
2017-01-01 05:01:00
@article{4403c4e0-08c4-44c7-856a-3152be74e9a0,
  abstract     = {Objective. The present experimental study explored pathomorphological changes and calcium depositions in the tympanic membrane during experimental acute otitis media caused by nontypeable Haemophilus influenzae in myringotomized and nonmyringotomized ears. Material and methods. A rat model of experimental acute otitis media caused by nontypeable Haemophilus influenzae was employed Sixteen Sprague-Dawley rats were used. Four days following middle ear inoculation, a bilateral myringotomy was performed in six randomly selected animals. Another group of 10 animals was inoculated only. On days 4, 7, 14, and 28, after inoculation, two animals from each group were sacrificed The temporal bones were removed and the tympanic membranes were dissected, followed by paraffin embedding. Adjacent sections were stained with PAS-alcian blue for basic histopathological observations and by von Kossa method for determination of calcium phosphate depositions. Results. Particularly intense invasion of polymorphonuclear neutrophil leukocytes was, seen on day 4 after inoculation. The highest infiltration of macrophages was observed on day 7. The peak number of lymphocytes was seen on day 14. No difference occurred in the number of polymorphonuclear leukocytes in myringotomized and nonmyringotomized tympanic membranes. The infiltration with lymphocytes and activated macrophages in all parts of the myringotomized tympanic membranes was statistically significantly higher than in the nonmyringotomized animals. The total amount of interstitial calcium phosphate depositions during days 7, 14, and 28 of study was statistically higher in the sections of pars tensa from myringotomized membranes compared to the nonmyringotomized membranes. Conclusion. Nontypeable Haemophilus influenzae-induced acute otitis media and myringotomy provoke more extensive inflammatory reaction with microcalcification in the tympanic membranes.},
  author       = {Alzbutiene, Giedre and Hermansson, Ann and Caye-Thomasen, Per and Kinduris, Vytenis},
  issn         = {1648-9144},
  keyword      = {myringosclerosis,acute otitis media,myringotomy,animal model},
  language     = {eng},
  number       = {4},
  pages        = {313--321},
  publisher    = {Kauno medicinos universitetas},
  series       = {Medicina},
  title        = {Tympanic membrane changes in experimental acute otitis media and myringotomy},
  volume       = {44},
  year         = {2008},
}