Advanced

Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters

Wiman, Magnus LU ; Andersson, Jonas and Lidén, Gunnar LU (2008) In Bioprocess and Biosystems Engineering 31(4). p.369-377
Abstract
A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration > 0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate > 0 g/L.h was found. Strategies for improving the co-substrate utilization by genetic engineering... (More)
A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration > 0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate > 0 g/L.h was found. Strategies for improving the co-substrate utilization by genetic engineering of the transport systems are furthermore suggested based on simulations. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cerevisiae, Saccharomyces, xylose uptake, jinetic modeling, hexose transporters
in
Bioprocess and Biosystems Engineering
volume
31
issue
4
pages
369 - 377
publisher
Springer
external identifiers
  • wos:000255998300012
  • scopus:44149113738
ISSN
1615-7605
DOI
10.1007/s00449-007-0169-1
language
English
LU publication?
yes
id
b058c0d3-c7ff-4c85-b559-f1853b58b13d (old id 1203529)
date added to LUP
2008-09-16 16:50:14
date last changed
2017-08-20 03:52:50
@article{b058c0d3-c7ff-4c85-b559-f1853b58b13d,
  abstract     = {A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration > 0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate > 0 g/L.h was found. Strategies for improving the co-substrate utilization by genetic engineering of the transport systems are furthermore suggested based on simulations.},
  author       = {Wiman, Magnus and Andersson, Jonas and Lidén, Gunnar},
  issn         = {1615-7605},
  keyword      = {cerevisiae,Saccharomyces,xylose uptake,jinetic modeling,hexose transporters},
  language     = {eng},
  number       = {4},
  pages        = {369--377},
  publisher    = {Springer},
  series       = {Bioprocess and Biosystems Engineering},
  title        = {Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters},
  url          = {http://dx.doi.org/10.1007/s00449-007-0169-1},
  volume       = {31},
  year         = {2008},
}