Protein crystals can be incommensurately modulated
(2008) In Journal of Applied Crystallography 41(Part 3). p.600-605- Abstract
- For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to... (More)
- For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to an 'actin ribbon' formed by the crystal lattice. Interestingly, the transition to the aperiodic state was shown to be reversible and the diffraction pattern returned to the periodic state during data collection. It is likely that the protein underwent a conformational change that affected the neighbouring profilin: actin molecules in such a way as to produce the observed modulation in the diffraction pattern. Future work will aim to trap the incommensurately modulated crystal state, for example using cryocooling or chemical crosslinking, thus allowing complete X-ray data to be collected. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1203680
- author
- Lovelace, Jeffrey J ; Murphy, Cameron R ; Daniels, Lee ; Narayan, Kartik ; Schutt, Clarence E ; Lindberg, Uno ; Svensson, Christer LU and Borgstahl, Gloria E. O.
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- modulated protein crystals, profilin, actin, modulated diffraction, satellite reflections, incommensurate crystallography
- in
- Journal of Applied Crystallography
- volume
- 41
- issue
- Part 3
- pages
- 600 - 605
- publisher
- International Union of Crystallography
- external identifiers
-
- wos:000255902400012
- scopus:43749101124
- ISSN
- 1600-5767
- DOI
- 10.1107/S0021889808010716
- language
- English
- LU publication?
- yes
- id
- 357b55cc-aa84-44b3-8eb9-71cc8acfe246 (old id 1203680)
- date added to LUP
- 2016-04-01 12:32:45
- date last changed
- 2022-01-27 06:33:04
@article{357b55cc-aa84-44b3-8eb9-71cc8acfe246,
abstract = {{For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to an 'actin ribbon' formed by the crystal lattice. Interestingly, the transition to the aperiodic state was shown to be reversible and the diffraction pattern returned to the periodic state during data collection. It is likely that the protein underwent a conformational change that affected the neighbouring profilin: actin molecules in such a way as to produce the observed modulation in the diffraction pattern. Future work will aim to trap the incommensurately modulated crystal state, for example using cryocooling or chemical crosslinking, thus allowing complete X-ray data to be collected.}},
author = {{Lovelace, Jeffrey J and Murphy, Cameron R and Daniels, Lee and Narayan, Kartik and Schutt, Clarence E and Lindberg, Uno and Svensson, Christer and Borgstahl, Gloria E. O.}},
issn = {{1600-5767}},
keywords = {{modulated protein crystals; profilin; actin; modulated diffraction; satellite reflections; incommensurate crystallography}},
language = {{eng}},
number = {{Part 3}},
pages = {{600--605}},
publisher = {{International Union of Crystallography}},
series = {{Journal of Applied Crystallography}},
title = {{Protein crystals can be incommensurately modulated}},
url = {{http://dx.doi.org/10.1107/S0021889808010716}},
doi = {{10.1107/S0021889808010716}},
volume = {{41}},
year = {{2008}},
}