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Protein crystals can be incommensurately modulated

Lovelace, Jeffrey J; Murphy, Cameron R; Daniels, Lee; Narayan, Kartik; Schutt, Clarence E; Lindberg, Uno; Svensson, Christer LU and Borgstahl, Gloria E. O. (2008) In Journal of Applied Crystallography 41(Part 3). p.600-605
Abstract
For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to... (More)
For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to an 'actin ribbon' formed by the crystal lattice. Interestingly, the transition to the aperiodic state was shown to be reversible and the diffraction pattern returned to the periodic state during data collection. It is likely that the protein underwent a conformational change that affected the neighbouring profilin: actin molecules in such a way as to produce the observed modulation in the diffraction pattern. Future work will aim to trap the incommensurately modulated crystal state, for example using cryocooling or chemical crosslinking, thus allowing complete X-ray data to be collected. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
modulated protein crystals, profilin, actin, modulated diffraction, satellite reflections, incommensurate crystallography
in
Journal of Applied Crystallography
volume
41
issue
Part 3
pages
600 - 605
publisher
Wiley-Blackwell
external identifiers
  • wos:000255902400012
  • scopus:43749101124
ISSN
1600-5767
DOI
10.1107/S0021889808010716
language
English
LU publication?
yes
id
357b55cc-aa84-44b3-8eb9-71cc8acfe246 (old id 1203680)
date added to LUP
2008-09-17 10:33:00
date last changed
2017-08-20 03:47:34
@article{357b55cc-aa84-44b3-8eb9-71cc8acfe246,
  abstract     = {For a normal periodic crystal, the X-ray diffraction pattern can be described by an orientation matrix and a set of three integers that indicate the reciprocal lattice points. Those integers determine the spacing along the reciprocal lattice directions. In aperiodic crystals, the diffraction pattern is modulated and the standard periodic main reflections are surrounded by satellite reflections. The successful indexing and refinement of the main unit cell and q vector using TWINSOLVE, developed by Svensson [(2003). Lund University, Sweden], are reported here for an incommensurately modulated, aperiodic crystal of a profilin: actin complex. The indexing showed that the modulation is along the b direction in the crystal, which corresponds to an 'actin ribbon' formed by the crystal lattice. Interestingly, the transition to the aperiodic state was shown to be reversible and the diffraction pattern returned to the periodic state during data collection. It is likely that the protein underwent a conformational change that affected the neighbouring profilin: actin molecules in such a way as to produce the observed modulation in the diffraction pattern. Future work will aim to trap the incommensurately modulated crystal state, for example using cryocooling or chemical crosslinking, thus allowing complete X-ray data to be collected.},
  author       = {Lovelace, Jeffrey J and Murphy, Cameron R and Daniels, Lee and Narayan, Kartik and Schutt, Clarence E and Lindberg, Uno and Svensson, Christer and Borgstahl, Gloria E. O.},
  issn         = {1600-5767},
  keyword      = {modulated protein crystals,profilin,actin,modulated diffraction,satellite reflections,incommensurate crystallography},
  language     = {eng},
  number       = {Part 3},
  pages        = {600--605},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Applied Crystallography},
  title        = {Protein crystals can be incommensurately modulated},
  url          = {http://dx.doi.org/10.1107/S0021889808010716},
  volume       = {41},
  year         = {2008},
}