Beta-microseminoprotein in serum correlates with the levels in seminal plasma of young, healthy males
Valtonen-André, Camilla LU ; Sävblom, Charlotta LU ; Fernlund, Per LU ; Lilja, Hans LU
; Giwercman, Aleksander
LU
and Lundwall, Åke
LU
(2008)
In Journal of Andrology
29(3).
p.330-337
- Abstract
- Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile,... (More)
- Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P <.001). Significant correlations were also found in seminal plasma between MSP and prostate-specific antigen (PSA) (r =.65, P <.001) and between MSP and Zn2+ (r =.54, P <.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the prostate secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of prostate secretion, despite the contribution from extra prostatic tissues. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1205266
- author
- Valtonen-André, Camilla
LU
; Sävblom, Charlotta
LU
; Fernlund, Per
LU
; Lilja, Hans
LU
; Giwercman, Aleksander
LU
and Lundwall, Åke
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immunoassay, expression, prostate, semen, purification
- in
- Journal of Andrology
- volume
- 29
- issue
- 3
- pages
- 330 - 337
- publisher
- American Society of Andrology
- external identifiers
-
- wos:000255339500010
- scopus:43449098575
- pmid:18222915
- ISSN
- 0196-3635
- DOI
- 10.2164/jandrol.107.003616
- language
- English
- LU publication?
- yes
- id
- 561daea0-eeda-4258-9ef2-878ae2776228 (old id 1205266)
- date added to LUP
- 2016-04-01 13:35:50
- date last changed
- 2022-03-14 00:52:47
@article{561daea0-eeda-4258-9ef2-878ae2776228,
abstract = {{Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P <.001). Significant correlations were also found in seminal plasma between MSP and prostate-specific antigen (PSA) (r =.65, P <.001) and between MSP and Zn2+ (r =.54, P <.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the prostate secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of prostate secretion, despite the contribution from extra prostatic tissues.}},
author = {{Valtonen-André, Camilla and Sävblom, Charlotta and Fernlund, Per and Lilja, Hans and Giwercman, Aleksander and Lundwall, Åke}},
issn = {{0196-3635}},
keywords = {{immunoassay; expression; prostate; semen; purification}},
language = {{eng}},
number = {{3}},
pages = {{330--337}},
publisher = {{American Society of Andrology}},
series = {{Journal of Andrology}},
title = {{Beta-microseminoprotein in serum correlates with the levels in seminal plasma of young, healthy males}},
url = {{http://dx.doi.org/10.2164/jandrol.107.003616}},
doi = {{10.2164/jandrol.107.003616}},
volume = {{29}},
year = {{2008}},
}