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Beta-microseminoprotein in serum correlates with the levels in seminal plasma of young, healthy males

Valtonen-André, Camilla LU ; Sävblom, Charlotta LU ; Fernlund, Per LU ; Lilja, Hans LU ; Giwercman, Aleksander LU and Lundwall, Åke LU (2008) In Journal of Andrology 29(3). p.330-337
Abstract
Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile,... (More)
Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P <.001). Significant correlations were also found in seminal plasma between MSP and prostate-specific antigen (PSA) (r =.65, P <.001) and between MSP and Zn2+ (r =.54, P <.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the prostate secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of prostate secretion, despite the contribution from extra prostatic tissues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunoassay, expression, prostate, semen, purification
in
Journal of Andrology
volume
29
issue
3
pages
330 - 337
publisher
American Society of Andrology
external identifiers
  • wos:000255339500010
  • scopus:43449098575
ISSN
0196-3635
DOI
10.2164/jandrol.107.003616
language
English
LU publication?
yes
id
561daea0-eeda-4258-9ef2-878ae2776228 (old id 1205266)
date added to LUP
2008-09-18 14:01:06
date last changed
2017-05-07 03:53:04
@article{561daea0-eeda-4258-9ef2-878ae2776228,
  abstract     = {Beta-microseminoprotein (MSP) is one of the most abundant proteins secreted by the prostate gland. Because MSP is also synthesized in nonreproductive organs, the establishment of a solid relationship between the levels of MSP in serum and semen is crucial for future studies connecting MSP with aging or diseases of the prostate gland. We developed a specific, competitive, europium-based immunoassay to measure MSP in serum and seminal plasma. We also produced recombinant MSP in insect cells using baculo virus and purified it to homogeneity by a novel approach with ethanol extraction and gel filtration. The median values of MSP in 205 young men were 12 mu g/L (2.5-97.5 percentile, 4.9-26 mu g/L) in serum and 0.53 g/L (2.5-97.5 percentile, 0.13-2.0 g/L) or 1.8 mg (2.5-97.5 percentile, 0.32-6.6 mg) in seminal plasma. MSP in serum showed significant correlation to MSP in seminal plasma (r =.50, P &lt;.001). Significant correlations were also found in seminal plasma between MSP and prostate-specific antigen (PSA) (r =.65, P &lt;.001) and between MSP and Zn2+ (r =.54, P &lt;.001). The yield of recombinant MSP in culture medium was 35 mg/L or higher, and recovery following ethanol extraction was 80%-90%. MSP in serum reflects the prostate secretion of MSP, and correlations were also found in seminal plasma between MSP and PSA and Zn2+. This suggests that MSP in serum can be used as a marker of prostate secretion, despite the contribution from extra prostatic tissues.},
  author       = {Valtonen-André, Camilla and Sävblom, Charlotta and Fernlund, Per and Lilja, Hans and Giwercman, Aleksander and Lundwall, Åke},
  issn         = {0196-3635},
  keyword      = {immunoassay,expression,prostate,semen,purification},
  language     = {eng},
  number       = {3},
  pages        = {330--337},
  publisher    = {American Society of Andrology},
  series       = {Journal of Andrology},
  title        = {Beta-microseminoprotein in serum correlates with the levels in seminal plasma of young, healthy males},
  url          = {http://dx.doi.org/10.2164/jandrol.107.003616},
  volume       = {29},
  year         = {2008},
}