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Immersed solid phase microextraction to measure chemical activity of lipophilic organic contaminants in fatty tissue samples

Ossiander, Lia; Reichenberg, Fredrik LU ; McLachlan, Michael S. and Mayer, Philipp (2008) In Chemosphere 71(8). p.1502-1510
Abstract
It is known that solid phase microextraction (SPME) fibers can be equilibrated directly within environmental matrices such as water, sediment and soil slurries. Here it is shown that this method can also be applied to biological tissue. SPME extraction of biological matrices reportedly causes lipophilic fouling of the fiber. However, we found no significant measurement bias when combining equilibrium sampling with fiber surface cleaning. The uptake of lipophilic organic pollutants from the tissue and into the SPME fiber coating was characterized by fast equilibrium partitioning without sample depletion and without impacting the sorptive properties of the fiber. The precision of the method when applied to hexachlorobenzene and several PCB... (More)
It is known that solid phase microextraction (SPME) fibers can be equilibrated directly within environmental matrices such as water, sediment and soil slurries. Here it is shown that this method can also be applied to biological tissue. SPME extraction of biological matrices reportedly causes lipophilic fouling of the fiber. However, we found no significant measurement bias when combining equilibrium sampling with fiber surface cleaning. The uptake of lipophilic organic pollutants from the tissue and into the SPME fiber coating was characterized by fast equilibrium partitioning without sample depletion and without impacting the sorptive properties of the fiber. The precision of the method when applied to hexachlorobenzene and several PCB congeners in harbor porpoise blubber was 15%, which includes the variation between SPME samplings, manual injections and the instrumental analysis. A good correlation (r(2) = 0.95) was obtained between SPME measurements of PCB 153 in blubber and concentrations obtained via a traditional analytical approach. These results indicate that SPME is a promising technique for measuring chemical activity in biological tissue, which would make it a useful tool for studying chemical distribution in organisms as well as biodilution and biomagnification phenomena. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
equilibrium sampling, PDMS, SPME, ESD
in
Chemosphere
volume
71
issue
8
pages
1502 - 1510
publisher
Elsevier
external identifiers
  • wos:000255384300012
  • scopus:41149161559
ISSN
1879-1298
DOI
10.1016/j.chemosphere.2007.11.060
language
English
LU publication?
yes
id
63a5b6c0-b32d-4c35-9e68-5704417605c5 (old id 1205324)
date added to LUP
2008-09-18 14:17:16
date last changed
2017-10-22 03:48:37
@article{63a5b6c0-b32d-4c35-9e68-5704417605c5,
  abstract     = {It is known that solid phase microextraction (SPME) fibers can be equilibrated directly within environmental matrices such as water, sediment and soil slurries. Here it is shown that this method can also be applied to biological tissue. SPME extraction of biological matrices reportedly causes lipophilic fouling of the fiber. However, we found no significant measurement bias when combining equilibrium sampling with fiber surface cleaning. The uptake of lipophilic organic pollutants from the tissue and into the SPME fiber coating was characterized by fast equilibrium partitioning without sample depletion and without impacting the sorptive properties of the fiber. The precision of the method when applied to hexachlorobenzene and several PCB congeners in harbor porpoise blubber was 15%, which includes the variation between SPME samplings, manual injections and the instrumental analysis. A good correlation (r(2) = 0.95) was obtained between SPME measurements of PCB 153 in blubber and concentrations obtained via a traditional analytical approach. These results indicate that SPME is a promising technique for measuring chemical activity in biological tissue, which would make it a useful tool for studying chemical distribution in organisms as well as biodilution and biomagnification phenomena.},
  author       = {Ossiander, Lia and Reichenberg, Fredrik and McLachlan, Michael S. and Mayer, Philipp},
  issn         = {1879-1298},
  keyword      = {equilibrium sampling,PDMS,SPME,ESD},
  language     = {eng},
  number       = {8},
  pages        = {1502--1510},
  publisher    = {Elsevier},
  series       = {Chemosphere},
  title        = {Immersed solid phase microextraction to measure chemical activity of lipophilic organic contaminants in fatty tissue samples},
  url          = {http://dx.doi.org/10.1016/j.chemosphere.2007.11.060},
  volume       = {71},
  year         = {2008},
}