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Pearson correlation analysis of micro-array data allows for the identification of genetic targets for early B-cell factor.

Månsson, Robert LU ; Tsapogas, Panagiotis LU ; Åkerlund, Mikael LU ; Lagergren, Anna LU ; Gisler, Ramiro LU and Sigvardsson, Mikael LU (2004) In Journal of Biological Chemistry 279(17). p.17905-17913
Abstract
B lymphocyte development is a complex biological process critically dependent on the transcription factor early B cell factor (EBF). To deepen understanding of the roles for EBF in this process, we have used Pearson correlation analysis to evaluate microarray data from a set of mouse B lymphoid cell lines representing different stages of development. Comparing the expression pattern of EBF to that of the other genes in the data set revealed that VpreB1, mb-1, and lambda5, all known target genes, presented high correlation values to EBF. High correlations were also seen for the VpreB3 and CD19 genes and biochemical as well as functional data supported that they are target genes for EBF even though the expression of CD19 was critically... (More)
B lymphocyte development is a complex biological process critically dependent on the transcription factor early B cell factor (EBF). To deepen understanding of the roles for EBF in this process, we have used Pearson correlation analysis to evaluate microarray data from a set of mouse B lymphoid cell lines representing different stages of development. Comparing the expression pattern of EBF to that of the other genes in the data set revealed that VpreB1, mb-1, and lambda5, all known target genes, presented high correlation values to EBF. High correlations were also seen for the VpreB3 and CD19 genes and biochemical as well as functional data supported that they are target genes for EBF even though the expression of CD19 was critically dependent of Pax-5. We also obtained evidence for extensive collaborative actions of EBF and E47 even though microarray analysis of hematopoetic progenitor cells ectopically expressing these proteins suggested that they activated only a subset of pre-B cell restricted genes. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
279
issue
17
pages
17905 - 17913
publisher
ASBMB
external identifiers
  • wos:000220870400124
  • scopus:2342441324
ISSN
1083-351X
DOI
10.1074/jbc.M400589200
language
English
LU publication?
yes
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d2ef8aba-6cd8-4725-925c-0b72fb11c664 (old id 120598)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14960572&dopt=Abstract
date added to LUP
2007-07-18 11:28:58
date last changed
2017-09-03 03:44:42
@article{d2ef8aba-6cd8-4725-925c-0b72fb11c664,
  abstract     = {B lymphocyte development is a complex biological process critically dependent on the transcription factor early B cell factor (EBF). To deepen understanding of the roles for EBF in this process, we have used Pearson correlation analysis to evaluate microarray data from a set of mouse B lymphoid cell lines representing different stages of development. Comparing the expression pattern of EBF to that of the other genes in the data set revealed that VpreB1, mb-1, and lambda5, all known target genes, presented high correlation values to EBF. High correlations were also seen for the VpreB3 and CD19 genes and biochemical as well as functional data supported that they are target genes for EBF even though the expression of CD19 was critically dependent of Pax-5. We also obtained evidence for extensive collaborative actions of EBF and E47 even though microarray analysis of hematopoetic progenitor cells ectopically expressing these proteins suggested that they activated only a subset of pre-B cell restricted genes.},
  author       = {Månsson, Robert and Tsapogas, Panagiotis and Åkerlund, Mikael and Lagergren, Anna and Gisler, Ramiro and Sigvardsson, Mikael},
  issn         = {1083-351X},
  language     = {eng},
  number       = {17},
  pages        = {17905--17913},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Pearson correlation analysis of micro-array data allows for the identification of genetic targets for early B-cell factor.},
  url          = {http://dx.doi.org/10.1074/jbc.M400589200},
  volume       = {279},
  year         = {2004},
}