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Optical responses, permeability and diol-specific reactivity of thin polyacrylamide gels containing immobilized phenylboronic acid

Kuzimenkova, Marina LU ; Ivanov, Alexander LU ; Thammakhet, Chongdee; Mikhalovska, Lyubov I.; Galaev, Igor LU ; Thavarungkul, Panote; Kanatharana, Proespichaya and Mattiasson, Bo LU (2008) In Polymer 49(6). p.1444-1454
Abstract
Thin semitransparent gels were prepared by radical copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) and acrylamide (AAm) taken in molar ratios from 8:92 to 16:84, respectively, in water. The gels were characterized by the content of immobilized NAAPBA and monomer conversion. Scanning electron microscopy revealed the micrometer size pores in the dried gels. The wet gels displayed a linear optical response to sugars with sensitivity decreasing in the series: D-fructose, D-galactose, D-glucose, D-mannose, N-acetyl-D-glucosamine in the sugar concentration range from 1 to 40-60 mM at pH 7.3. Cross-linking of the gels with N,N-methylene-bis-acrylamide decreased the strength of optical response. Specific binding capacity of a... (More)
Thin semitransparent gels were prepared by radical copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) and acrylamide (AAm) taken in molar ratios from 8:92 to 16:84, respectively, in water. The gels were characterized by the content of immobilized NAAPBA and monomer conversion. Scanning electron microscopy revealed the micrometer size pores in the dried gels. The wet gels displayed a linear optical response to sugars with sensitivity decreasing in the series: D-fructose, D-galactose, D-glucose, D-mannose, N-acetyl-D-glucosamine in the sugar concentration range from 1 to 40-60 mM at pH 7.3. Cross-linking of the gels with N,N-methylene-bis-acrylamide decreased the strength of optical response. Specific binding capacity of a diol-containing dye Alizarin Red S in the gels at pH 7.0 coincided with the content of immobilized NAAPBA indicating the 1: 1 stoichiometry of the reaction and, therefore, good accessibility of the boronic acid ligands for water-soluble diols. Permeability of the gels was studied with a non-interacting dye Ethyl Orange exhibiting the pore diffusion coefficient of 1.4 x 10(-7) cm(2)/s. The rate of optical response of the gels to glucose was found to be determined by diffusion of sugar into the relatively thick gels (1 = 0.35-1 mm) with effective diffusion coefficients of 2 x 10(-7) cm(2)/S. In the thinner gels (l = 0.1 mm) the input of other kinetic processes, such as affinity binding or structural rearrangements of the gel, was noticeable. (c) 2008 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
boronic acid, hydrogels, sugar sensing
in
Polymer
volume
49
issue
6
pages
1444 - 1454
publisher
Elsevier
external identifiers
  • wos:000254818900004
  • scopus:40249091260
ISSN
0032-3861
DOI
10.1016/j.polymer.2008.02.009
language
English
LU publication?
yes
id
f47db3d4-ac94-4ea0-9b10-2b854c6b6138 (old id 1206596)
date added to LUP
2008-08-28 14:45:00
date last changed
2017-01-01 05:56:18
@article{f47db3d4-ac94-4ea0-9b10-2b854c6b6138,
  abstract     = {Thin semitransparent gels were prepared by radical copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) and acrylamide (AAm) taken in molar ratios from 8:92 to 16:84, respectively, in water. The gels were characterized by the content of immobilized NAAPBA and monomer conversion. Scanning electron microscopy revealed the micrometer size pores in the dried gels. The wet gels displayed a linear optical response to sugars with sensitivity decreasing in the series: D-fructose, D-galactose, D-glucose, D-mannose, N-acetyl-D-glucosamine in the sugar concentration range from 1 to 40-60 mM at pH 7.3. Cross-linking of the gels with N,N-methylene-bis-acrylamide decreased the strength of optical response. Specific binding capacity of a diol-containing dye Alizarin Red S in the gels at pH 7.0 coincided with the content of immobilized NAAPBA indicating the 1: 1 stoichiometry of the reaction and, therefore, good accessibility of the boronic acid ligands for water-soluble diols. Permeability of the gels was studied with a non-interacting dye Ethyl Orange exhibiting the pore diffusion coefficient of 1.4 x 10(-7) cm(2)/s. The rate of optical response of the gels to glucose was found to be determined by diffusion of sugar into the relatively thick gels (1 = 0.35-1 mm) with effective diffusion coefficients of 2 x 10(-7) cm(2)/S. In the thinner gels (l = 0.1 mm) the input of other kinetic processes, such as affinity binding or structural rearrangements of the gel, was noticeable. (c) 2008 Elsevier Ltd. All rights reserved.},
  author       = {Kuzimenkova, Marina and Ivanov, Alexander and Thammakhet, Chongdee and Mikhalovska, Lyubov I. and Galaev, Igor and Thavarungkul, Panote and Kanatharana, Proespichaya and Mattiasson, Bo},
  issn         = {0032-3861},
  keyword      = {boronic acid,hydrogels,sugar sensing},
  language     = {eng},
  number       = {6},
  pages        = {1444--1454},
  publisher    = {Elsevier},
  series       = {Polymer},
  title        = {Optical responses, permeability and diol-specific reactivity of thin polyacrylamide gels containing immobilized phenylboronic acid},
  url          = {http://dx.doi.org/10.1016/j.polymer.2008.02.009},
  volume       = {49},
  year         = {2008},
}