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Comparison of ethyl glucuronide in hair with phosphatidylethanol in whole blood as post-mortem markers of alcohol abuse

Bendroth, Peter; Kronstrand, Robert; Helander, Anders; Greby, Jesper; Stephanson, Nikolai and Krantz, Peter LU (2008) In Forensic Science International 176(1). p.76-81
Abstract
Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been used as a marker of alcohol abuse in both urine and hair. This study investigated the value of EtG testing in post-mortem hair for diagnostic improvement of alcohol abuse in forensic medicine. Material from 70 consecutive medico-legal autopsies was collected in accordance with the recommendations on ethics by the Swedish National Board of Forensic Medicine. A method for determination of EtG in hair samples was developed using ultra performance liquid chromatography/electrospray tandem mass spectrometry (UPLC/ESI-MS/MS; LOQ, 2.5 pg/mg). The result of the EtG analysis was compared with the findings of phosphatidylethanol (PEth) in femoral whole blood, as measured by high... (More)
Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been used as a marker of alcohol abuse in both urine and hair. This study investigated the value of EtG testing in post-mortem hair for diagnostic improvement of alcohol abuse in forensic medicine. Material from 70 consecutive medico-legal autopsies was collected in accordance with the recommendations on ethics by the Swedish National Board of Forensic Medicine. A method for determination of EtG in hair samples was developed using ultra performance liquid chromatography/electrospray tandem mass spectrometry (UPLC/ESI-MS/MS; LOQ, 2.5 pg/mg). The result of the EtG analysis was compared with the findings of phosphatidylethanol (PEth) in femoral whole blood, as measured by high performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD; LOQ, 0.22 mu mol/l). Evaluation of liver histology and anamnestic evidence of alcohol abuse of the deceased were taken in consideration for the interpretation. Measurable levels of EtG were present in 49 of the 70 autopsy cases whereas PEth was present in 36. Thirty-nine cases had EtG levels above the cutoff limit (>= 30 pg/mg) compared with 29 for PEth (>= 0.7 mu mol/l). Fifteen cases had FIG as exclusive indicator for alcohol abuse compared with four cases for PEth. These findings suggest that measurements of EtG in hair may provide improved diagnostic information on alcohol abuse. due to a long retrospective time-window for detection and stability of EtG in hair in the decaying cadaver. However, an EtG level below the cutoff does not completely exclude previous alcohol abuse. (C) 2007 Elsevier Ireland Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alcohol marker, phosphatidylethanol, ethyl glucuronide, hair, forensic, medicine
in
Forensic Science International
volume
176
issue
1
pages
76 - 81
publisher
Elsevier
external identifiers
  • wos:000254789600016
  • scopus:39449129609
ISSN
1872-6283
DOI
10.1016/j.forsciint.2007.09.012
language
English
LU publication?
yes
id
f902de86-3bdd-43c9-bd01-3da9a462202c (old id 1207245)
date added to LUP
2008-08-28 13:47:49
date last changed
2017-09-24 03:33:56
@article{f902de86-3bdd-43c9-bd01-3da9a462202c,
  abstract     = {Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been used as a marker of alcohol abuse in both urine and hair. This study investigated the value of EtG testing in post-mortem hair for diagnostic improvement of alcohol abuse in forensic medicine. Material from 70 consecutive medico-legal autopsies was collected in accordance with the recommendations on ethics by the Swedish National Board of Forensic Medicine. A method for determination of EtG in hair samples was developed using ultra performance liquid chromatography/electrospray tandem mass spectrometry (UPLC/ESI-MS/MS; LOQ, 2.5 pg/mg). The result of the EtG analysis was compared with the findings of phosphatidylethanol (PEth) in femoral whole blood, as measured by high performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD; LOQ, 0.22 mu mol/l). Evaluation of liver histology and anamnestic evidence of alcohol abuse of the deceased were taken in consideration for the interpretation. Measurable levels of EtG were present in 49 of the 70 autopsy cases whereas PEth was present in 36. Thirty-nine cases had EtG levels above the cutoff limit (>= 30 pg/mg) compared with 29 for PEth (>= 0.7 mu mol/l). Fifteen cases had FIG as exclusive indicator for alcohol abuse compared with four cases for PEth. These findings suggest that measurements of EtG in hair may provide improved diagnostic information on alcohol abuse. due to a long retrospective time-window for detection and stability of EtG in hair in the decaying cadaver. However, an EtG level below the cutoff does not completely exclude previous alcohol abuse. (C) 2007 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Bendroth, Peter and Kronstrand, Robert and Helander, Anders and Greby, Jesper and Stephanson, Nikolai and Krantz, Peter},
  issn         = {1872-6283},
  keyword      = {alcohol marker,phosphatidylethanol,ethyl glucuronide,hair,forensic,medicine},
  language     = {eng},
  number       = {1},
  pages        = {76--81},
  publisher    = {Elsevier},
  series       = {Forensic Science International},
  title        = {Comparison of ethyl glucuronide in hair with phosphatidylethanol in whole blood as post-mortem markers of alcohol abuse},
  url          = {http://dx.doi.org/10.1016/j.forsciint.2007.09.012},
  volume       = {176},
  year         = {2008},
}