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Rapid and efficient clearance of airway tissue granulocytes through transepithelial migration.

Erjefält, Jonas LU ; Uller, Lena LU ; Malm-Erjefält, Monika LU and Persson, Carl LU (2004) In Thorax 59(2). p.136-143
Abstract
Background: Clearance of tissue granulocytes is central to the resolution of airway inflammation. To date the focus has been on apoptotic mechanisms of cell removal and little attention has been given to alternative processes. The present study explores transepithelial migration as a mechanism of cell clearance.



Method: Guinea pig tracheobronchial airways where eosinophils are constitutively present in the mucosal tissue were studied. A complex topical stimulus (allergen challenge) was applied and the fate of the eosinophils was determined by selective tracheobronchial lavage and histological examination of the tissue.



Results: Within 10 minutes of the allergen challenge, massive migration of... (More)
Background: Clearance of tissue granulocytes is central to the resolution of airway inflammation. To date the focus has been on apoptotic mechanisms of cell removal and little attention has been given to alternative processes. The present study explores transepithelial migration as a mechanism of cell clearance.



Method: Guinea pig tracheobronchial airways where eosinophils are constitutively present in the mucosal tissue were studied. A complex topical stimulus (allergen challenge) was applied and the fate of the eosinophils was determined by selective tracheobronchial lavage and histological examination of the tissue.



Results: Within 10 minutes of the allergen challenge, massive migration of eosinophils into the airway lumen occurred together with a reduction in tissue eosinophil numbers. Cell clearance into the lumen continued at high speed and by 30 and 60 minutes the tissue eosinophilia had been reduced by 63% and 73%, respectively. The marked transepithelial migration (estimated maximal speed 35 000 cells/min x cm2 mucosal surface) took place ubiquitously between epithelial cells without affecting epithelial integrity as assessed by transmission and scanning electron microscopy. Eosinophil apoptosis was not detected but occasional cytolytic eosinophils occurred.



Conclusion: This study shows that luminal entry has a remarkably high capacity as a granulocyte elimination process. The data also suggest that an appropriate stimulus of transepithelial migration may be used therapeutically to increase the resolution of inflammatory conditions of airway tissues. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Thorax
volume
59
issue
2
pages
136 - 143
publisher
BMJ Publishing Group
external identifiers
  • wos:000188750200013
  • scopus:10744220329
ISSN
1468-3296
DOI
10.1136/thorax.2003.004218
language
English
LU publication?
yes
id
e12d9804-5c92-45d0-b362-4de6941dd203 (old id 120779)
alternative location
http://thorax.bmjjournals.com/cgi/content/full/59/2/136
date added to LUP
2007-07-12 08:42:03
date last changed
2017-08-27 05:36:15
@article{e12d9804-5c92-45d0-b362-4de6941dd203,
  abstract     = {Background: Clearance of tissue granulocytes is central to the resolution of airway inflammation. To date the focus has been on apoptotic mechanisms of cell removal and little attention has been given to alternative processes. The present study explores transepithelial migration as a mechanism of cell clearance.<br/><br>
<br/><br>
Method: Guinea pig tracheobronchial airways where eosinophils are constitutively present in the mucosal tissue were studied. A complex topical stimulus (allergen challenge) was applied and the fate of the eosinophils was determined by selective tracheobronchial lavage and histological examination of the tissue.<br/><br>
<br/><br>
Results: Within 10 minutes of the allergen challenge, massive migration of eosinophils into the airway lumen occurred together with a reduction in tissue eosinophil numbers. Cell clearance into the lumen continued at high speed and by 30 and 60 minutes the tissue eosinophilia had been reduced by 63% and 73%, respectively. The marked transepithelial migration (estimated maximal speed 35 000 cells/min x cm2 mucosal surface) took place ubiquitously between epithelial cells without affecting epithelial integrity as assessed by transmission and scanning electron microscopy. Eosinophil apoptosis was not detected but occasional cytolytic eosinophils occurred.<br/><br>
<br/><br>
Conclusion: This study shows that luminal entry has a remarkably high capacity as a granulocyte elimination process. The data also suggest that an appropriate stimulus of transepithelial migration may be used therapeutically to increase the resolution of inflammatory conditions of airway tissues.},
  author       = {Erjefält, Jonas and Uller, Lena and Malm-Erjefält, Monika and Persson, Carl},
  issn         = {1468-3296},
  language     = {eng},
  number       = {2},
  pages        = {136--143},
  publisher    = {BMJ Publishing Group},
  series       = {Thorax},
  title        = {Rapid and efficient clearance of airway tissue granulocytes through transepithelial migration.},
  url          = {http://dx.doi.org/10.1136/thorax.2003.004218},
  volume       = {59},
  year         = {2004},
}