Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis
(2008) In Journal of Bacteriology 190(13). p.4660-4665- Abstract
- The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of... (More)
- The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1209077
- author
- Carlsson Möller, Mirja LU and Hederstedt, Lars LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 190
- issue
- 13
- pages
- 4660 - 4665
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000257174400032
- scopus:46049117838
- pmid:18456801
- ISSN
- 0021-9193
- DOI
- 10.1128/JB.00252-08
- language
- English
- LU publication?
- yes
- id
- 4f6465de-1a1c-4ae3-8a89-56b19940f8c9 (old id 1209077)
- date added to LUP
- 2016-04-01 12:17:07
- date last changed
- 2022-01-27 01:28:36
@article{4f6465de-1a1c-4ae3-8a89-56b19940f8c9, abstract = {{The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane.}}, author = {{Carlsson Möller, Mirja and Hederstedt, Lars}}, issn = {{0021-9193}}, language = {{eng}}, number = {{13}}, pages = {{4660--4665}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Bacteriology}}, title = {{Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in <em>Bacillus subtilis</em>}}, url = {{http://dx.doi.org/10.1128/JB.00252-08}}, doi = {{10.1128/JB.00252-08}}, volume = {{190}}, year = {{2008}}, }