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Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis

Carlsson Möller, Mirja LU and Hederstedt, Lars LU (2008) In Journal of Bacteriology 190(13). p.4660-4665
Abstract
The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of... (More)
The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
190
issue
13
pages
4660 - 4665
publisher
American Society for Microbiology
external identifiers
  • wos:000257174400032
  • scopus:46049117838
ISSN
0021-9193
DOI
10.1128/JB.00252-08
language
English
LU publication?
yes
id
4f6465de-1a1c-4ae3-8a89-56b19940f8c9 (old id 1209077)
date added to LUP
2008-08-25 13:56:37
date last changed
2017-08-09 15:59:50
@article{4f6465de-1a1c-4ae3-8a89-56b19940f8c9,
  abstract     = {The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane.},
  author       = {Carlsson Möller, Mirja and Hederstedt, Lars},
  issn         = {0021-9193},
  language     = {eng},
  number       = {13},
  pages        = {4660--4665},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in <em>Bacillus subtilis</em>},
  url          = {http://dx.doi.org/10.1128/JB.00252-08},
  volume       = {190},
  year         = {2008},
}