Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L.
(2000) In Plant Science 154(1). p.53-60- Abstract
- The seed oil in Macadamia integrifolia contains about 30% palmitoleic acid (16:1(Delta9)) and Nerium oleander about 12% isoricinoleic acid (Delta9-hydroxy-18:1(Delta12)). It has been shown that palmitoleic acid can be produced by acyl-acyl carrier protein (ACP) desaturases and it has also been shown that fatty acid hydroxylation can occur via direct substitution of a hydrogen atom. Therefore it seemed possible that the enzymes responsible for the making of these unusual fatty acids in M. integrifolia and N. oleander were of acyl-ACP desaturase type. Extracts from developing M. integrifolia developing seeds showed a relative ratio of 16:0-ACP to 18:0-ACP desaturation that was about 13 times higher than in sunflower seeds. N. oleander seed... (More)
- The seed oil in Macadamia integrifolia contains about 30% palmitoleic acid (16:1(Delta9)) and Nerium oleander about 12% isoricinoleic acid (Delta9-hydroxy-18:1(Delta12)). It has been shown that palmitoleic acid can be produced by acyl-acyl carrier protein (ACP) desaturases and it has also been shown that fatty acid hydroxylation can occur via direct substitution of a hydrogen atom. Therefore it seemed possible that the enzymes responsible for the making of these unusual fatty acids in M. integrifolia and N. oleander were of acyl-ACP desaturase type. Extracts from developing M. integrifolia developing seeds showed a relative ratio of 16:0-ACP to 18:0-ACP desaturation that was about 13 times higher than in sunflower seeds. N. oleander seed extracts catalysed conversion of 18:0-ACP to 18:1(Delta9) but only trace amounts of Delta9-hydroxy fatty acids were formed. A total of four cDNAs were isolated from developing seeds, of both species, using a fragment isolated with PCR amplification. The M. integrifolia acyl-ACP desaturase cDNA was expressed in Escherichia coli. A partly purified fraction of the enzyme showed a 16:0-ACP to 18:0-ACP desaturation ratio about 90-fold less than that in the Macadamia extracts. Expressed N. oleander acyl-ACP desaturase cDNAs showed predominantly 18:0-ACP desaturase activity and no hydroxylase activity. Thus it is not likely that any of the four acyl-ACP desaturases cloned from M. integrifolia or N. oleander is involved in the production of unusual fatty acids. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1209911
- author
- Gummeson, PO ; Lenman, Marit LU ; Lee, M ; Singh, S and Stymne, S
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Macadamia integrifolia, Acyl-ACP desaturase, Nerium oleander
- in
- Plant Science
- volume
- 154
- issue
- 1
- pages
- 53 - 60
- publisher
- Elsevier
- external identifiers
-
- scopus:0034657829
- ISSN
- 0168-9452
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100)
- id
- 58712c34-d4e9-4633-85a1-e509c136ed63 (old id 1209911)
- alternative location
- http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TBH-3YTJCPX-6-5&_cdi=5143&_user=745831&_orig=browse&_coverDate=05%2F15%2F2000&_sk=998459998&view=c&wchp=dGLbVzz-zSkzk&md5=c850997313adfee883744034a3b2de49&ie=/sdarticle.pdf
- date added to LUP
- 2016-04-01 16:35:10
- date last changed
- 2022-01-28 20:44:52
@article{58712c34-d4e9-4633-85a1-e509c136ed63,
abstract = {{The seed oil in Macadamia integrifolia contains about 30% palmitoleic acid (16:1(Delta9)) and Nerium oleander about 12% isoricinoleic acid (Delta9-hydroxy-18:1(Delta12)). It has been shown that palmitoleic acid can be produced by acyl-acyl carrier protein (ACP) desaturases and it has also been shown that fatty acid hydroxylation can occur via direct substitution of a hydrogen atom. Therefore it seemed possible that the enzymes responsible for the making of these unusual fatty acids in M. integrifolia and N. oleander were of acyl-ACP desaturase type. Extracts from developing M. integrifolia developing seeds showed a relative ratio of 16:0-ACP to 18:0-ACP desaturation that was about 13 times higher than in sunflower seeds. N. oleander seed extracts catalysed conversion of 18:0-ACP to 18:1(Delta9) but only trace amounts of Delta9-hydroxy fatty acids were formed. A total of four cDNAs were isolated from developing seeds, of both species, using a fragment isolated with PCR amplification. The M. integrifolia acyl-ACP desaturase cDNA was expressed in Escherichia coli. A partly purified fraction of the enzyme showed a 16:0-ACP to 18:0-ACP desaturation ratio about 90-fold less than that in the Macadamia extracts. Expressed N. oleander acyl-ACP desaturase cDNAs showed predominantly 18:0-ACP desaturase activity and no hydroxylase activity. Thus it is not likely that any of the four acyl-ACP desaturases cloned from M. integrifolia or N. oleander is involved in the production of unusual fatty acids.}},
author = {{Gummeson, PO and Lenman, Marit and Lee, M and Singh, S and Stymne, S}},
issn = {{0168-9452}},
keywords = {{Macadamia integrifolia; Acyl-ACP desaturase; Nerium oleander}},
language = {{eng}},
number = {{1}},
pages = {{53--60}},
publisher = {{Elsevier}},
series = {{Plant Science}},
title = {{Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L.}},
url = {{http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TBH-3YTJCPX-6-5&_cdi=5143&_user=745831&_orig=browse&_coverDate=05%2F15%2F2000&_sk=998459998&view=c&wchp=dGLbVzz-zSkzk&md5=c850997313adfee883744034a3b2de49&ie=/sdarticle.pdf}},
volume = {{154}},
year = {{2000}},
}