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Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation

Lee, M; Lenman, Marit LU ; Banas, A; Bafor, M; Singh, S; Schweizer, M; Nilsson, R; Liljenberg, C; Dahlqvist, A and Gummeson, PO, et al. (1998) In Science 280(5365). p.915-918
Abstract
Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.
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type
Contribution to journal
publication status
published
subject
in
Science
volume
280
issue
5365
pages
915 - 918
publisher
The American Association for the Advancement of Science
external identifiers
  • scopus:0032496331
ISSN
1095-9203
DOI
10.1126/science.280.5365.915
language
English
LU publication?
no
id
2ce2d33c-c589-4a91-ad2b-bca1f3794150 (old id 1209922)
date added to LUP
2008-08-25 14:20:25
date last changed
2017-10-29 04:22:19
@article{2ce2d33c-c589-4a91-ad2b-bca1f3794150,
  abstract     = {Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.},
  author       = {Lee, M and Lenman, Marit and Banas, A and Bafor, M and Singh, S and Schweizer, M and Nilsson, R and Liljenberg, C and Dahlqvist, A and Gummeson, PO and Sjödahl, S and Green, A and Stymne, S},
  issn         = {1095-9203},
  language     = {eng},
  number       = {5365},
  pages        = {915--918},
  publisher    = {The American Association for the Advancement of Science},
  series       = {Science},
  title        = {Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation},
  url          = {http://dx.doi.org/10.1126/science.280.5365.915},
  volume       = {280},
  year         = {1998},
}