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The kinetics of DNA-cationic vesicle complex formation

Barreleiro, Paula LU and Lindman, Björn LU (2003) In The Journal of Physical Chemistry Part B 107(25). p.6208-6213
Abstract
Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly ads

rbs onto the oppositely charged membrane surface (milliseconds), and

thereafter, on a... (More)
Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly ads

rbs onto the oppositely charged membrane surface (milliseconds), and

thereafter, on a longer time scale (seconds), the formed DNA-lipid

complex continues to aggregate and grow; a third step is also observed,

which seems to be due to rearrangement of the complex with a further

change of the DNA conformation. The time constant of each step was

determined by the relative amounts of DNA and cationic vesicles and the

composition of the lipid bilayer of the

vesicles. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
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in
The Journal of Physical Chemistry Part B
volume
107
issue
25
pages
6208 - 6213
publisher
The American Chemical Society
external identifiers
  • wos:000183649500027
  • scopus:0038756339
ISSN
1520-5207
DOI
language
English
LU publication?
yes
id
1c80ebb7-699c-4edd-84a1-7e92c15e6268 (old id 121939)
date added to LUP
2007-07-10 17:06:50
date last changed
2018-06-24 04:38:18
@article{1c80ebb7-699c-4edd-84a1-7e92c15e6268,
  abstract     = {Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly ads<br/><br>
rbs onto the oppositely charged membrane surface (milliseconds), and<br/><br>
	thereafter, on a longer time scale (seconds), the formed DNA-lipid<br/><br>
	complex continues to aggregate and grow; a third step is also observed,<br/><br>
	which seems to be due to rearrangement of the complex with a further<br/><br>
	change of the DNA conformation. The time constant of each step was<br/><br>
	determined by the relative amounts of DNA and cationic vesicles and the<br/><br>
	composition of the lipid bilayer of the<br/><br>
	vesicles.},
  author       = {Barreleiro, Paula and Lindman, Björn},
  issn         = {1520-5207},
  language     = {eng},
  number       = {25},
  pages        = {6208--6213},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {The kinetics of DNA-cationic vesicle complex formation},
  url          = {http://dx.doi.org/},
  volume       = {107},
  year         = {2003},
}