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Gemini surfactants: New synthetic vectors for gene transfection

Kirby, A J; Camilleri, P; Engberts, J; Feiters, M. C.; Nolte, R J M; Söderman, Olle LU ; Bergsma, M; Bell, P C; Fielden, M L and Rodriguez, C L G, et al. (2003) In Angewandte Chemie: International Edition in English1962-01-01+01:001997-01-01+01:00 42(13). p.1448-1457
Abstract
The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the... (More)
The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency. (Less)
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published
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in
Angewandte Chemie: International Edition in English1962-01-01+01:001997-01-01+01:00
volume
42
issue
13
pages
1448 - 1457
publisher
John Wiley & Sons
external identifiers
  • wos:000182337000003
  • pmid:12698476
  • scopus:0345352681
ISSN
0570-0833
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language
English
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yes
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960ecbd5-3abe-438b-82a2-ad73abf08b64 (old id 122051)
date added to LUP
2007-07-11 14:13:38
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2018-05-29 11:30:35
@article{960ecbd5-3abe-438b-82a2-ad73abf08b64,
  abstract     = {The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.},
  author       = {Kirby, A J and Camilleri, P and Engberts, J and Feiters, M. C. and Nolte, R J M and Söderman, Olle and Bergsma, M and Bell, P C and Fielden, M L and Rodriguez, C L G and Guedat, P and Kremer, A and McGregor, C and Perrin, C and Ronsin, G and van Eijk, Marcel C P},
  issn         = {0570-0833},
  language     = {eng},
  number       = {13},
  pages        = {1448--1457},
  publisher    = {John Wiley & Sons},
  series       = {Angewandte Chemie: International Edition in English1962-01-01+01:001997-01-01+01:00},
  title        = {Gemini surfactants: New synthetic vectors for gene transfection},
  url          = {http://dx.doi.org/},
  volume       = {42},
  year         = {2003},
}