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Standardization of Cystatin C: development of primary and secondary reference preparations.

Blirup, Soren LU ; Grubb, Anders LU orcid ; Lindström, Veronica LU orcid ; Schmidt, C and Althaus, H (2008) In Scandinavian Journal of Clinical & Laboratory Investigation 68(s241). p.67-70
Abstract
A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Primary Reference Preparation, Secondary Reference Preparation, standardization of Cystatin C, dry mass determination, Cystatin C
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
68
issue
s241
pages
67 - 70
publisher
Informa Healthcare
external identifiers
  • wos:000256682700012
  • pmid:18569968
  • scopus:45849097552
ISSN
1502-7686
DOI
10.1080/00365510802150067
language
English
LU publication?
yes
id
e5973629-86fa-4955-bf67-5d6a17d8f23a (old id 1223070)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18569968?dopt=Abstract
date added to LUP
2016-04-01 12:51:08
date last changed
2023-01-11 07:07:04
@article{e5973629-86fa-4955-bf67-5d6a17d8f23a,
  abstract     = {{A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.}},
  author       = {{Blirup, Soren and Grubb, Anders and Lindström, Veronica and Schmidt, C and Althaus, H}},
  issn         = {{1502-7686}},
  keywords     = {{Primary Reference Preparation; Secondary Reference Preparation; standardization of Cystatin C; dry mass determination; Cystatin C}},
  language     = {{eng}},
  number       = {{s241}},
  pages        = {{67--70}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical & Laboratory Investigation}},
  title        = {{Standardization of Cystatin C: development of primary and secondary reference preparations.}},
  url          = {{http://dx.doi.org/10.1080/00365510802150067}},
  doi          = {{10.1080/00365510802150067}},
  volume       = {{68}},
  year         = {{2008}},
}