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Production of polyhydroxyalkanoates by glycogen accumulating organisms treating a paper mill wastewater.

Bengtsson, Simon LU ; Werker, Alan and Welander, Thomas (2008) In Water Science and Technology 58(2). p.323-330
Abstract
A process for production of polyhydroxyalkanoates (PHA) by activated sludge treating a paper mill wastewater was investigated. The applied strategy was to select for glycogen accumulating organisms (GAOs) by alternating anaerobic/aerobic conditions. Acidogenic fermentation was used as pretreatment to convert various organic compounds to volatile fatty acids which are preferable substrates for PHA production. Enrichment resulted in a culture dominated by GAOs related to Defluviicoccus vanus (56%) and Candidatus Competibacter phosphatis (22%). Optimization of PHA accumulation by the enriched GAO culture was performed through batch experiments. Accumulation of PHA under anaerobic conditions was limited by the intracellular glycogen stored.... (More)
A process for production of polyhydroxyalkanoates (PHA) by activated sludge treating a paper mill wastewater was investigated. The applied strategy was to select for glycogen accumulating organisms (GAOs) by alternating anaerobic/aerobic conditions. Acidogenic fermentation was used as pretreatment to convert various organic compounds to volatile fatty acids which are preferable substrates for PHA production. Enrichment resulted in a culture dominated by GAOs related to Defluviicoccus vanus (56%) and Candidatus Competibacter phosphatis (22%). Optimization of PHA accumulation by the enriched GAO culture was performed through batch experiments. Accumulation of PHA under anaerobic conditions was limited by the intracellular glycogen stored. Under aerobic conditions significant glycogen production (to 25% of sludge dry weight) was observed alongside PHA accumulation (to 22% of sludge dry weight). By applying a subsequent anaerobic period after an initial aerobic, the produced glycogen could be utilized for further PHA accumulation and by this strategy PHA content was increased to 42% of sludge dry weight. The PHA yield over the entire process was 0.10 kg per kg of influent COD treated which is similar to what has been achieved with a process applying feast/famine enrichment strategy with the same wastewater. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polyhydroxyalkanoates (PHA), Defluviicoccus vanus, competibacter phosphatis, metabolism, glycogen accumulating organisms (GAO), mixed culture, paper mill wastewater
in
Water Science and Technology
volume
58
issue
2
pages
323 - 330
publisher
IWA Publishing
external identifiers
  • wos:000258485600008
  • pmid:18701781
  • scopus:52949126364
ISSN
0273-1223
DOI
10.2166/wst.2008.381
language
English
LU publication?
yes
id
9c2c34b5-f34b-4a6b-8c79-1162d4e74511 (old id 1223243)
date added to LUP
2008-10-16 16:14:06
date last changed
2017-11-19 04:02:56
@article{9c2c34b5-f34b-4a6b-8c79-1162d4e74511,
  abstract     = {A process for production of polyhydroxyalkanoates (PHA) by activated sludge treating a paper mill wastewater was investigated. The applied strategy was to select for glycogen accumulating organisms (GAOs) by alternating anaerobic/aerobic conditions. Acidogenic fermentation was used as pretreatment to convert various organic compounds to volatile fatty acids which are preferable substrates for PHA production. Enrichment resulted in a culture dominated by GAOs related to Defluviicoccus vanus (56%) and Candidatus Competibacter phosphatis (22%). Optimization of PHA accumulation by the enriched GAO culture was performed through batch experiments. Accumulation of PHA under anaerobic conditions was limited by the intracellular glycogen stored. Under aerobic conditions significant glycogen production (to 25% of sludge dry weight) was observed alongside PHA accumulation (to 22% of sludge dry weight). By applying a subsequent anaerobic period after an initial aerobic, the produced glycogen could be utilized for further PHA accumulation and by this strategy PHA content was increased to 42% of sludge dry weight. The PHA yield over the entire process was 0.10 kg per kg of influent COD treated which is similar to what has been achieved with a process applying feast/famine enrichment strategy with the same wastewater.},
  author       = {Bengtsson, Simon and Werker, Alan and Welander, Thomas},
  issn         = {0273-1223},
  keyword      = {polyhydroxyalkanoates (PHA),Defluviicoccus vanus,competibacter phosphatis,metabolism,glycogen accumulating organisms (GAO),mixed culture,paper mill wastewater},
  language     = {eng},
  number       = {2},
  pages        = {323--330},
  publisher    = {IWA Publishing},
  series       = {Water Science and Technology},
  title        = {Production of polyhydroxyalkanoates by glycogen accumulating organisms treating a paper mill wastewater.},
  url          = {http://dx.doi.org/10.2166/wst.2008.381},
  volume       = {58},
  year         = {2008},
}