Exit of pediatric pre-B acute lymphoblastic leukaemia cells from the bone marrow to the peripheral blood is not associated with cell maturation or alterations in gene expression.
(2008) In Molecular Cancer 7.- Abstract
- BACKGROUND: Childhood pre-B acute lymphoblastic leukemia (ALL) is a bone marrow (BM) derived disease, which often disseminates out of the BM cavity, where malignant cells to a variable degree can be found circulating in the peripheral blood (PB). Normal pre-B cells are absolutely dependent on BM stroma for survival and differentiation. It is not known whether transformed pre-B ALL cells retain any of this dependence, which possibly could impact on drug sensitivity or MRD measurements. RESULTS: Pre-B ALL cells, highly purified by a novel method using surface expression of CD19 and immunoglobulin light chains, from BM and PB show a very high degree of similarity in gene expression patterns, with differential expression of vascular... (More)
- BACKGROUND: Childhood pre-B acute lymphoblastic leukemia (ALL) is a bone marrow (BM) derived disease, which often disseminates out of the BM cavity, where malignant cells to a variable degree can be found circulating in the peripheral blood (PB). Normal pre-B cells are absolutely dependent on BM stroma for survival and differentiation. It is not known whether transformed pre-B ALL cells retain any of this dependence, which possibly could impact on drug sensitivity or MRD measurements. RESULTS: Pre-B ALL cells, highly purified by a novel method using surface expression of CD19 and immunoglobulin light chains, from BM and PB show a very high degree of similarity in gene expression patterns, with differential expression of vascular endothelial growth factor (VEGF) as a notable exception. In addition, the cell sorting procedure revealed that in 2 out of five investigated patients, a significant fraction of the malignant cells had matured beyond the pre-B cell stage. CONCLUSION: The transition of ALL cells from the BM into the circulation does not demand, or result in, major changes of gene expression pattern. This might indicate an independence of BM stroma on the part of transformed pre-B cells, which contrasts with that of their normal counterparts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1223287
- author
- Hansson, Frida ; Toporski, Jacek LU ; Månsson, Robert LU ; Johansson, Bertil LU ; Norén-Nyström, Ulrika ; Jacobsen, Sten Eirik W LU ; Wiebe, Thomas LU ; Larsson, Marcus LU ; Sigvardsson, Mikael LU and Castor, Anders LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Cancer
- volume
- 7
- article number
- 67
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000258958600001
- pmid:18694513
- scopus:50649098470
- pmid:18694513
- ISSN
- 1476-4598
- DOI
- 10.1186/1476-4598-7-67
- language
- English
- LU publication?
- yes
- id
- 77836718-7183-4458-b60d-17fd27bb8f17 (old id 1223287)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18694513?dopt=Abstract
- date added to LUP
- 2016-04-04 09:42:19
- date last changed
- 2022-01-29 19:10:25
@article{77836718-7183-4458-b60d-17fd27bb8f17,
abstract = {{BACKGROUND: Childhood pre-B acute lymphoblastic leukemia (ALL) is a bone marrow (BM) derived disease, which often disseminates out of the BM cavity, where malignant cells to a variable degree can be found circulating in the peripheral blood (PB). Normal pre-B cells are absolutely dependent on BM stroma for survival and differentiation. It is not known whether transformed pre-B ALL cells retain any of this dependence, which possibly could impact on drug sensitivity or MRD measurements. RESULTS: Pre-B ALL cells, highly purified by a novel method using surface expression of CD19 and immunoglobulin light chains, from BM and PB show a very high degree of similarity in gene expression patterns, with differential expression of vascular endothelial growth factor (VEGF) as a notable exception. In addition, the cell sorting procedure revealed that in 2 out of five investigated patients, a significant fraction of the malignant cells had matured beyond the pre-B cell stage. CONCLUSION: The transition of ALL cells from the BM into the circulation does not demand, or result in, major changes of gene expression pattern. This might indicate an independence of BM stroma on the part of transformed pre-B cells, which contrasts with that of their normal counterparts.}},
author = {{Hansson, Frida and Toporski, Jacek and Månsson, Robert and Johansson, Bertil and Norén-Nyström, Ulrika and Jacobsen, Sten Eirik W and Wiebe, Thomas and Larsson, Marcus and Sigvardsson, Mikael and Castor, Anders}},
issn = {{1476-4598}},
language = {{eng}},
publisher = {{BioMed Central (BMC)}},
series = {{Molecular Cancer}},
title = {{Exit of pediatric pre-B acute lymphoblastic leukaemia cells from the bone marrow to the peripheral blood is not associated with cell maturation or alterations in gene expression.}},
url = {{http://dx.doi.org/10.1186/1476-4598-7-67}},
doi = {{10.1186/1476-4598-7-67}},
volume = {{7}},
year = {{2008}},
}