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Different assessments of immunohistochemically stained Ki-67 and hTERT in glioblastoma multiforme yield variable results: a study with reference to survival prognosis.

Persson, Annette LU and Englund, Elisabet LU (2008) In Clinical Neuropathology 27(4). p.224-233
Abstract
OBJECTIVE: To investigate a marker of tumor proliferation, Ki-67, and telomerase expression in glioblastoma multiforme and to compare the results of different mainly quantitative assessments, in relation to age and survival rates. METHODS: Immunohistochemical stainings of Ki-67 and hTERT were evaluated in 39 formaldehyde-fixed, paraffin-embedded surgical samples of glioblastoma multiforme diagnosed during 2004, comprising all specimens with sufficient amount of vital tissue sent to the Department of Pathology during this year. Ki-67 counting and hTERT evaluation was assessed on whole tumor sections and on selected areas within each section. Age and length of survival were analyzed in relation to these parameters. RESULTS: We found that... (More)
OBJECTIVE: To investigate a marker of tumor proliferation, Ki-67, and telomerase expression in glioblastoma multiforme and to compare the results of different mainly quantitative assessments, in relation to age and survival rates. METHODS: Immunohistochemical stainings of Ki-67 and hTERT were evaluated in 39 formaldehyde-fixed, paraffin-embedded surgical samples of glioblastoma multiforme diagnosed during 2004, comprising all specimens with sufficient amount of vital tissue sent to the Department of Pathology during this year. Ki-67 counting and hTERT evaluation was assessed on whole tumor sections and on selected areas within each section. Age and length of survival were analyzed in relation to these parameters. RESULTS: We found that different methods of evaluating the stained sections yielded different results regarding Ki-67, but less marked differences for hTERT. With Ki-67 counting on whole sections (non-selected areas), we found a statistically significant correlation with length of survival. There was no corresponding information in the hTERT assessment. We could also confirm a significant inverse correlation between age and length of survival, as previously published. CONCLUSION: Our data demonstrate that different methods of Ki-67 evaluation may give markedly dissimilar results. The significant correlation found between survival and one but not with two other methods of Ki-67 assessment, implicate the value of standardized quantification methods. Our data indicate a possible prognostic use of immunohistochemical Ki-67 proliferation index in glioblastoma multiforme. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
Ki-67 Antigen: biosynthesis, Glioblastoma: pathology, Glioblastoma: mortality, Glioblastoma: metabolism, Brain Neoplasms: pathology, Brain Neoplasms: metabolism, Brain Neoplasms: mortality, Telomerase: biosynthesis, Tumor Markers, Biological: analysis
in
Clinical Neuropathology
volume
27
issue
4
pages
224 - 233
publisher
Dustri-Verlag
external identifiers
  • WOS:000257663800002
  • PMID:18666438
  • Scopus:47349129485
ISSN
0722-5091
language
English
LU publication?
yes
id
648c8bc2-67d8-432a-8f8a-cb3aae5da41b (old id 1223683)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18666438?dopt=Abstract
date added to LUP
2008-09-10 11:25:19
date last changed
2017-01-01 07:47:57
@article{648c8bc2-67d8-432a-8f8a-cb3aae5da41b,
  abstract     = {OBJECTIVE: To investigate a marker of tumor proliferation, Ki-67, and telomerase expression in glioblastoma multiforme and to compare the results of different mainly quantitative assessments, in relation to age and survival rates. METHODS: Immunohistochemical stainings of Ki-67 and hTERT were evaluated in 39 formaldehyde-fixed, paraffin-embedded surgical samples of glioblastoma multiforme diagnosed during 2004, comprising all specimens with sufficient amount of vital tissue sent to the Department of Pathology during this year. Ki-67 counting and hTERT evaluation was assessed on whole tumor sections and on selected areas within each section. Age and length of survival were analyzed in relation to these parameters. RESULTS: We found that different methods of evaluating the stained sections yielded different results regarding Ki-67, but less marked differences for hTERT. With Ki-67 counting on whole sections (non-selected areas), we found a statistically significant correlation with length of survival. There was no corresponding information in the hTERT assessment. We could also confirm a significant inverse correlation between age and length of survival, as previously published. CONCLUSION: Our data demonstrate that different methods of Ki-67 evaluation may give markedly dissimilar results. The significant correlation found between survival and one but not with two other methods of Ki-67 assessment, implicate the value of standardized quantification methods. Our data indicate a possible prognostic use of immunohistochemical Ki-67 proliferation index in glioblastoma multiforme.},
  author       = {Persson, Annette and Englund, Elisabet},
  issn         = {0722-5091},
  keyword      = {Ki-67 Antigen: biosynthesis,Glioblastoma: pathology,Glioblastoma: mortality,Glioblastoma: metabolism,Brain Neoplasms: pathology,Brain Neoplasms: metabolism,Brain Neoplasms: mortality,Telomerase: biosynthesis,Tumor Markers,Biological: analysis},
  language     = {eng},
  number       = {4},
  pages        = {224--233},
  publisher    = {Dustri-Verlag},
  series       = {Clinical Neuropathology},
  title        = {Different assessments of immunohistochemically stained Ki-67 and hTERT in glioblastoma multiforme yield variable results: a study with reference to survival prognosis.},
  volume       = {27},
  year         = {2008},
}