Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride.
(2009) In Bioresource Technology 100(3). p.1350-1357- Abstract
- Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation... (More)
- Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation broths were used instead of the supernatants, thus the mycelium bound enzymes were also present, the hydrolytic capacity of T. reesei Rut C30 was enhanced by approximately 200%, while an improvement of only 15% was observed in case of the T. atroviride isolates. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1242991
- author
- Kovacs, Krisztina LU ; Szakacs, George and Zacchi, Guido LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Trichoderma atroviride, Trichoderma reesei, Cellulase production, β-Glucosidase production, Enzymatic hydrolysis
- in
- Bioresource Technology
- volume
- 100
- issue
- 3
- pages
- 1350 - 1357
- publisher
- Elsevier
- external identifiers
-
- wos:000261569100048
- pmid:18793835
- scopus:55749110015
- pmid:18793835
- ISSN
- 1873-2976
- DOI
- 10.1016/j.biortech.2008.08.006
- language
- English
- LU publication?
- yes
- id
- b3a200fa-355b-43a0-9da9-0eb1240d102a (old id 1242991)
- date added to LUP
- 2016-04-01 13:59:01
- date last changed
- 2025-04-04 14:08:00
@article{b3a200fa-355b-43a0-9da9-0eb1240d102a,
abstract = {{Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation broths were used instead of the supernatants, thus the mycelium bound enzymes were also present, the hydrolytic capacity of T. reesei Rut C30 was enhanced by approximately 200%, while an improvement of only 15% was observed in case of the T. atroviride isolates.}},
author = {{Kovacs, Krisztina and Szakacs, George and Zacchi, Guido}},
issn = {{1873-2976}},
keywords = {{Trichoderma atroviride; Trichoderma reesei; Cellulase production; β-Glucosidase production; Enzymatic hydrolysis}},
language = {{eng}},
number = {{3}},
pages = {{1350--1357}},
publisher = {{Elsevier}},
series = {{Bioresource Technology}},
title = {{Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride.}},
url = {{http://dx.doi.org/10.1016/j.biortech.2008.08.006}},
doi = {{10.1016/j.biortech.2008.08.006}},
volume = {{100}},
year = {{2009}},
}