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Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride.

Kovacs, Krisztina LU ; Szakacs, George and Zacchi, Guido LU (2009) In Bioresource Technology 100(3). p.1350-1357
Abstract
Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation... (More)
Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation broths were used instead of the supernatants, thus the mycelium bound enzymes were also present, the hydrolytic capacity of T. reesei Rut C30 was enhanced by approximately 200%, while an improvement of only 15% was observed in case of the T. atroviride isolates. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Trichoderma atroviride, Trichoderma reesei, Cellulase production, β-Glucosidase production, Enzymatic hydrolysis
in
Bioresource Technology
volume
100
issue
3
pages
1350 - 1357
publisher
Elsevier
external identifiers
  • wos:000261569100048
  • pmid:18793835
  • scopus:55749110015
ISSN
1873-2976
DOI
10.1016/j.biortech.2008.08.006
language
English
LU publication?
yes
id
b3a200fa-355b-43a0-9da9-0eb1240d102a (old id 1242991)
date added to LUP
2008-10-13 12:28:27
date last changed
2017-09-24 04:02:49
@article{b3a200fa-355b-43a0-9da9-0eb1240d102a,
  abstract     = {Cellulase and beta-glucosidase production on steam pretreated spruce (SPS) with Trichoderma reesei Rut C30, Trichoderma atroviride TUB F-1505 and TUB F-1663 was investigated. The enzymes were compared in term of activity, temperature optima and hydrolytic capacity. The T. atroviride cellulases proved to have lower temperature optima for filter paper activity (FPA) assay (50 degrees C) and for hydrolysis of SPS (40 degrees C) than the Rut C30 enzymes (60 degrees C for FPA and 50 degrees C for hydrolysis). Due to high levels of extracellular beta-glucosidases, the T. atroviride enzyme supernatants hydrolyzed the washed SPS to glucose more efficiently than the enzymes produced by T. reesei. On the other hand, when the whole fermentation broths were used instead of the supernatants, thus the mycelium bound enzymes were also present, the hydrolytic capacity of T. reesei Rut C30 was enhanced by approximately 200%, while an improvement of only 15% was observed in case of the T. atroviride isolates.},
  author       = {Kovacs, Krisztina and Szakacs, George and Zacchi, Guido},
  issn         = {1873-2976},
  keyword      = {Trichoderma atroviride,Trichoderma reesei,Cellulase production,β-Glucosidase production,Enzymatic hydrolysis},
  language     = {eng},
  number       = {3},
  pages        = {1350--1357},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride.},
  url          = {http://dx.doi.org/10.1016/j.biortech.2008.08.006},
  volume       = {100},
  year         = {2009},
}