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Protein standardization V: value transfer. A practical protocol for the assignment of serum protein values from a Reference Material to a Target Material.

Blirup, Soren LU ; Johnson, A Myron and Larsen, Marianne (2008) In Clinical Chemistry and Laboratory Medicine Sep 1. p.1470-1479
Abstract
Abstract We present a practical protocol for the assignment of values to serum proteins in a Target Material using a Reference Material. This protocol is based on the model of Direct Value Transfer between serum matrices and is intended to improve the value assignment of commercial calibrators using the Reference Material CRM 470 (now labeled ERM-DA 470) or similar reference materials. The procedure describes the general as well as the practical principles involved in the value assignment (with examples). The practical transfer protocol is based on multiple assays of 6 dilutions of the Reference Material and 6 dilutions of the Target Material. The transfer protocol requires several measurements a day repeated on several days, an important... (More)
Abstract We present a practical protocol for the assignment of values to serum proteins in a Target Material using a Reference Material. This protocol is based on the model of Direct Value Transfer between serum matrices and is intended to improve the value assignment of commercial calibrators using the Reference Material CRM 470 (now labeled ERM-DA 470) or similar reference materials. The procedure describes the general as well as the practical principles involved in the value assignment (with examples). The practical transfer protocol is based on multiple assays of 6 dilutions of the Reference Material and 6 dilutions of the Target Material. The transfer protocol requires several measurements a day repeated on several days, an important prerequisite being that all reconstitutions and dilutions are controlled by weighing thus reducing uncertainty in the transfer. In open systems that allow the use of the Reference Material as calibrator and the Target Material as samples, the proportionality of the two materials (the presence or absence of matrix effects) can now be directly assessed by evaluating a single regression plot. If no matrix effects are found, the regression line will pass through zero with a slope equal to the ratio of the concentrations of the two materials. In closed systems, the dedicated commercial calibrator has to be used as such; the Reference Material and the Target Material are now assayed as samples against this calibrator. Two regression plots are therefore obtained; if no matrix effects are present among the two materials and the calibrator, both the Reference and Target Materials will show zero intercepts, and the ratio of the two slopes will equal the ratio of the concentrations. Clin Chem Lab Med 2008;46. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Chemistry and Laboratory Medicine
volume
Sep 1
pages
1470 - 1479
publisher
De Gruyter
external identifiers
  • wos:000259929400022
  • pmid:18761461
  • scopus:53849108778
ISSN
1434-6621
DOI
10.1515/CCLM.2008.289
language
English
LU publication?
yes
id
a3cef851-7838-4816-8814-824c39ff3b5a (old id 1243545)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18761461?dopt=Abstract
date added to LUP
2008-10-03 14:24:04
date last changed
2017-07-30 04:50:50
@article{a3cef851-7838-4816-8814-824c39ff3b5a,
  abstract     = {Abstract We present a practical protocol for the assignment of values to serum proteins in a Target Material using a Reference Material. This protocol is based on the model of Direct Value Transfer between serum matrices and is intended to improve the value assignment of commercial calibrators using the Reference Material CRM 470 (now labeled ERM-DA 470) or similar reference materials. The procedure describes the general as well as the practical principles involved in the value assignment (with examples). The practical transfer protocol is based on multiple assays of 6 dilutions of the Reference Material and 6 dilutions of the Target Material. The transfer protocol requires several measurements a day repeated on several days, an important prerequisite being that all reconstitutions and dilutions are controlled by weighing thus reducing uncertainty in the transfer. In open systems that allow the use of the Reference Material as calibrator and the Target Material as samples, the proportionality of the two materials (the presence or absence of matrix effects) can now be directly assessed by evaluating a single regression plot. If no matrix effects are found, the regression line will pass through zero with a slope equal to the ratio of the concentrations of the two materials. In closed systems, the dedicated commercial calibrator has to be used as such; the Reference Material and the Target Material are now assayed as samples against this calibrator. Two regression plots are therefore obtained; if no matrix effects are present among the two materials and the calibrator, both the Reference and Target Materials will show zero intercepts, and the ratio of the two slopes will equal the ratio of the concentrations. Clin Chem Lab Med 2008;46.},
  author       = {Blirup, Soren and Johnson, A Myron and Larsen, Marianne},
  issn         = {1434-6621},
  language     = {eng},
  pages        = {1470--1479},
  publisher    = {De Gruyter},
  series       = {Clinical Chemistry and Laboratory Medicine},
  title        = {Protein standardization V: value transfer. A practical protocol for the assignment of serum protein values from a Reference Material to a Target Material.},
  url          = {http://dx.doi.org/10.1515/CCLM.2008.289},
  volume       = {Sep 1},
  year         = {2008},
}