Advanced

Biosynthesis of selenoproteins in cultured bovine mammary cells.

Bruzelius, Katharina LU ; Purup, S; James, Peter LU ; Önning, Gunilla LU and Åkesson, Björn LU (2008) In Journal of Trace Elements in Medicine and Biology 22(3). p.224-233
Abstract
The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the... (More)
The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the system, the effect of retinoic acid (RA) on the formation of selenoproteins was investigated. The concentration of 75Se in total cell protein was reduced by about 35% in cells cultured with RA compared with control cells, while the opposite effect was observed in protein precipitated from cell culture medium, which contained 60% more 75Se in RA-treated samples than in controls. There were also indications that RA might affect different selenoproteins in different ways. The methods described provide a promising approach for further studies of the regulation of selenoprotein formation in the mammary gland. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Trace Elements in Medicine and Biology
volume
22
issue
3
pages
224 - 233
publisher
Elsevier
external identifiers
  • pmid:18755398
  • wos:000259810800007
  • scopus:49849095585
ISSN
1878-3252
DOI
10.1016/j.jtemb.2008.03.009
language
English
LU publication?
yes
id
7e63b2f8-aa2a-4b4f-bf58-8cc7d73b22cd (old id 1244427)
date added to LUP
2008-10-06 14:40:24
date last changed
2017-05-28 03:28:57
@article{7e63b2f8-aa2a-4b4f-bf58-8cc7d73b22cd,
  abstract     = {The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the system, the effect of retinoic acid (RA) on the formation of selenoproteins was investigated. The concentration of 75Se in total cell protein was reduced by about 35% in cells cultured with RA compared with control cells, while the opposite effect was observed in protein precipitated from cell culture medium, which contained 60% more 75Se in RA-treated samples than in controls. There were also indications that RA might affect different selenoproteins in different ways. The methods described provide a promising approach for further studies of the regulation of selenoprotein formation in the mammary gland.},
  author       = {Bruzelius, Katharina and Purup, S and James, Peter and Önning, Gunilla and Åkesson, Björn},
  issn         = {1878-3252},
  language     = {eng},
  number       = {3},
  pages        = {224--233},
  publisher    = {Elsevier},
  series       = {Journal of Trace Elements in Medicine and Biology},
  title        = {Biosynthesis of selenoproteins in cultured bovine mammary cells.},
  url          = {http://dx.doi.org/10.1016/j.jtemb.2008.03.009},
  volume       = {22},
  year         = {2008},
}