Biosynthesis of selenoproteins in cultured bovine mammary cells.
Bruzelius, Katharina LU ; Purup, S ; James, Peter LU
; Önning, Gunilla
LU
and Åkesson, Björn
LU
(2008)
In Journal of Trace Elements in Medicine and Biology
22(3).
p.224-233
- Abstract
- The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the... (More)
- The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the system, the effect of retinoic acid (RA) on the formation of selenoproteins was investigated. The concentration of 75Se in total cell protein was reduced by about 35% in cells cultured with RA compared with control cells, while the opposite effect was observed in protein precipitated from cell culture medium, which contained 60% more 75Se in RA-treated samples than in controls. There were also indications that RA might affect different selenoproteins in different ways. The methods described provide a promising approach for further studies of the regulation of selenoprotein formation in the mammary gland. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1244427
- author
- Bruzelius, Katharina
LU
; Purup, S
; James, Peter
LU
; Önning, Gunilla
LU
and Åkesson, Björn
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Trace Elements in Medicine and Biology
- volume
- 22
- issue
- 3
- pages
- 224 - 233
- publisher
- Elsevier
- external identifiers
-
- pmid:18755398
- wos:000259810800007
- scopus:49849095585
- pmid:18755398
- ISSN
- 1878-3252
- DOI
- 10.1016/j.jtemb.2008.03.009
- language
- English
- LU publication?
- yes
- id
- 7e63b2f8-aa2a-4b4f-bf58-8cc7d73b22cd (old id 1244427)
- date added to LUP
- 2016-04-01 11:35:23
- date last changed
- 2023-08-31 23:37:29
@article{7e63b2f8-aa2a-4b4f-bf58-8cc7d73b22cd,
abstract = {{The biosynthesis of selenoproteins was studied in relation to milk formation and mammary cell biology by incubating the bovine mammary cell line MAC-T with (75Se)selenite. Intracellular proteins and proteins secreted into the cell culture medium were separated by 2D electrophoresis, the selenoproteins were detected by autoradiography, and the proteins were identified by MALDI-TOF. Approximately 35 75Se-containing spots were found in the cell proteins from MAC-T cells. Among them, one-third showed high intensity. The strongest spot was identified as glutathione peroxidase 1. About 20 spots were observed in protein precipitated from cell culture medium, one-third of them being distinctly visible. In an attempt to study a perturbation of the system, the effect of retinoic acid (RA) on the formation of selenoproteins was investigated. The concentration of 75Se in total cell protein was reduced by about 35% in cells cultured with RA compared with control cells, while the opposite effect was observed in protein precipitated from cell culture medium, which contained 60% more 75Se in RA-treated samples than in controls. There were also indications that RA might affect different selenoproteins in different ways. The methods described provide a promising approach for further studies of the regulation of selenoprotein formation in the mammary gland.}},
author = {{Bruzelius, Katharina and Purup, S and James, Peter and Önning, Gunilla and Åkesson, Björn}},
issn = {{1878-3252}},
language = {{eng}},
number = {{3}},
pages = {{224--233}},
publisher = {{Elsevier}},
series = {{Journal of Trace Elements in Medicine and Biology}},
title = {{Biosynthesis of selenoproteins in cultured bovine mammary cells.}},
url = {{http://dx.doi.org/10.1016/j.jtemb.2008.03.009}},
doi = {{10.1016/j.jtemb.2008.03.009}},
volume = {{22}},
year = {{2008}},
}