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CD4+ T-cells have a key instructive role in educating dendritic cells in allergy

Larsson, Kristina LU ; Lindstedt, Malin LU ; Lundberg, Kristina LU ; Dexlin Mellby, Linda LU ; Wingren, Christer LU ; Korsgren, Magnus LU ; Greiff, Lennart LU and Borrebaeck, Carl LU (2009) In International Archives of Allergy and Immunology1994-01-01+01:00 149(1). p.1-15
Abstract
Background: Dendritic cells (DCs) are central in allergy as regulators of the Th1/Th2 balance. We have recently demonstrated a unique transcriptional profile of DCs in patients with ongoing allergy compared with healthy subjects and shown that crosstalk between DCs and memory T cells affects the transcriptional profile of T cells. However, the transcriptional profile of DCs educated by T cells in allergy is unknown. Methods: In the present study, we have examined the transcriptional profiles of DCs after stimulation with grass pollen allergens, Phleum pratense and coculture with autologous CD4+ memory T cells using high-density microarray. Protein analysis was performed using flow cytometry and recombinant antibody protein microarrays.... (More)
Background: Dendritic cells (DCs) are central in allergy as regulators of the Th1/Th2 balance. We have recently demonstrated a unique transcriptional profile of DCs in patients with ongoing allergy compared with healthy subjects and shown that crosstalk between DCs and memory T cells affects the transcriptional profile of T cells. However, the transcriptional profile of DCs educated by T cells in allergy is unknown. Methods: In the present study, we have examined the transcriptional profiles of DCs after stimulation with grass pollen allergens, Phleum pratense and coculture with autologous CD4+ memory T cells using high-density microarray. Protein analysis was performed using flow cytometry and recombinant antibody protein microarrays. Patients with allergic rhinitis and healthy subjects were compared. Results: The results reveal a distinct T-cell-induced DC profile in atopic individuals. Accordingly, about 170 genes were upregulated and 40 genes downregulated. For example, the chemokine receptor CXCR4 and the tumor necrosis factor receptor CD30 were upregulated in DCs derived from atopic donors, and this could also be verified at the protein level. Conclusion: We conclude that crosstalk between CD4+ memory T cells and autologous DCs induces transcriptional reprogramming in DCs. This finding suggests that T cells have a key instructive role in educating DCs in Th2-type responses. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Archives of Allergy and Immunology1994-01-01+01:00
volume
149
issue
1
pages
1 - 15
publisher
Karger
external identifiers
  • pmid:19033727
  • wos:000262422900001
  • scopus:56449131005
ISSN
1423-0097
DOI
10.1159/000176301
language
English
LU publication?
yes
id
e178f49b-7de2-4258-93a2-649326e95dcc (old id 1245693)
date added to LUP
2008-10-13 11:17:02
date last changed
2017-01-01 04:50:51
@article{e178f49b-7de2-4258-93a2-649326e95dcc,
  abstract     = {Background: Dendritic cells (DCs) are central in allergy as regulators of the Th1/Th2 balance. We have recently demonstrated a unique transcriptional profile of DCs in patients with ongoing allergy compared with healthy subjects and shown that crosstalk between DCs and memory T cells affects the transcriptional profile of T cells. However, the transcriptional profile of DCs educated by T cells in allergy is unknown. Methods: In the present study, we have examined the transcriptional profiles of DCs after stimulation with grass pollen allergens, Phleum pratense and coculture with autologous CD4+ memory T cells using high-density microarray. Protein analysis was performed using flow cytometry and recombinant antibody protein microarrays. Patients with allergic rhinitis and healthy subjects were compared. Results: The results reveal a distinct T-cell-induced DC profile in atopic individuals. Accordingly, about 170 genes were upregulated and 40 genes downregulated. For example, the chemokine receptor CXCR4 and the tumor necrosis factor receptor CD30 were upregulated in DCs derived from atopic donors, and this could also be verified at the protein level. Conclusion: We conclude that crosstalk between CD4+ memory T cells and autologous DCs induces transcriptional reprogramming in DCs. This finding suggests that T cells have a key instructive role in educating DCs in Th2-type responses.},
  author       = {Larsson, Kristina and Lindstedt, Malin and Lundberg, Kristina and Dexlin Mellby, Linda and Wingren, Christer and Korsgren, Magnus and Greiff, Lennart and Borrebaeck, Carl},
  issn         = {1423-0097},
  language     = {eng},
  number       = {1},
  pages        = {1--15},
  publisher    = {Karger},
  series       = {International Archives of Allergy and Immunology1994-01-01+01:00},
  title        = {CD4+ T-cells have a key instructive role in educating dendritic cells in allergy},
  url          = {http://dx.doi.org/10.1159/000176301},
  volume       = {149},
  year         = {2009},
}