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Serum response factor is required for sprouting angiogenesis and vascular integrity

Franco, Claudio Areias; Mericskay, Mathias; Parlakian, Ara; Gary-Bobo, Guillaume; Gao-Li, Jacqueline; Paulin, Denise; Gustafsson, Erika LU and Li, Zhenlin (2008) In Developmental Cell 15(3). p.448-461
Abstract
Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and beta-actin in ECs both in vivo and in vitro. Moreover,... (More)
Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and beta-actin in ECs both in vivo and in vitro. Moreover, knockdown of SRF in ECs impaired VEGF- and FGF-induced in vitro angiogenesis. Taken together, our results demonstrate that SRF plays an important role in sprouting angiogenesis and small vessel integrity in the mouse embryo. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Developmental Cell
volume
15
issue
3
pages
448 - 461
publisher
Cell Press
external identifiers
  • wos:000259358100015
  • scopus:51449099892
ISSN
1534-5807
DOI
10.1016/j.devcel.2008.07.019
language
English
LU publication?
yes
id
31239897-89f7-4076-a489-42ce3153a8ea (old id 1246700)
date added to LUP
2008-11-17 15:55:03
date last changed
2017-11-05 03:33:29
@article{31239897-89f7-4076-a489-42ce3153a8ea,
  abstract     = {Serum response factor (SRF) is a transcription factor that controls the expression of cytoskeletal proteins and immediate early genes in different cell types. Here, we found that SRF expression is restricted to endothelial cells (ECs) of small vessels such as capillaries in the mouse embryo. EC-specific Srf deletion led to aneurysms and hemorrhages from 11.5 days of mouse development (E11.5) and lethality at E14.5. Mutant embryos presented a reduced capillary density and defects in EC migration, with fewer numbers of filopodia in tip cells and ECs showing defects in actin polymerization and intercellular junctions. We show that SRF is essential for the expression of VE-cadherin and beta-actin in ECs both in vivo and in vitro. Moreover, knockdown of SRF in ECs impaired VEGF- and FGF-induced in vitro angiogenesis. Taken together, our results demonstrate that SRF plays an important role in sprouting angiogenesis and small vessel integrity in the mouse embryo.},
  author       = {Franco, Claudio Areias and Mericskay, Mathias and Parlakian, Ara and Gary-Bobo, Guillaume and Gao-Li, Jacqueline and Paulin, Denise and Gustafsson, Erika and Li, Zhenlin},
  issn         = {1534-5807},
  language     = {eng},
  number       = {3},
  pages        = {448--461},
  publisher    = {Cell Press},
  series       = {Developmental Cell},
  title        = {Serum response factor is required for sprouting angiogenesis and vascular integrity},
  url          = {http://dx.doi.org/10.1016/j.devcel.2008.07.019},
  volume       = {15},
  year         = {2008},
}