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Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers

Kumar, Ashok LU ; Wahlund, Per-Olof LU ; Kepka, Cecilia LU ; Galaev, Igor LU and Mattiasson, Bo LU (2003) In Biotechnology and Bioengineering 84(4). p.494-503
Abstract
Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions. The present lack of widespread applications for this technique as compared to immobilized metal affinity chromatography (IMAC) may be related to the scarcity of well-characterized metal affinity macroligands (AML) and their applications to the number of different purification systems. In the present work we describe a detailed study of a new purification system using metal-loaded thermoresponsive copolymers as AML. The copolymers of vinylimidazole (VI) with N-isopropylacrylamide (NIPAM) were synthesized by radical polymerization with imidazole contents of 15 and 24 mol%. When loaded... (More)
Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions. The present lack of widespread applications for this technique as compared to immobilized metal affinity chromatography (IMAC) may be related to the scarcity of well-characterized metal affinity macroligands (AML) and their applications to the number of different purification systems. In the present work we describe a detailed study of a new purification system using metal-loaded thermoresponsive copolymers as AML. The copolymers of vinylimidazole (VI) with N-isopropylacrylamide (NIPAM) were synthesized by radical polymerization with imidazole contents of 15 and 24 mol%. When loaded with Cu(II) and Ni(II) ions the copolymers selectively precipitated extracellularly expressed histidine-tagged single-chain Fv-antibody fragments (His6-scFv fragments) from the fermentation broth free from E. coli cells. Precipitation was induced by salt at mild temperatures and the bound antibody fragments were recovered by dissolving the protein-polymer complex in EDTA buffer and subsequent reprecipitation of the polymer. His6-scFv fragments were purified with yields of 91 and 80% and purification folds of 16 and 21 when Cu(II) and Ni(II) copolymers were used, respectively. The protein precipitation capacity of the Ni(II) copolymer showed a dependence on the VI concentration in the copolymer. The SDS-PAGE pattern showed significant purification of the antibody fragments. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 84: 494-503, 2003. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
metal chelate affinity precipitation, His/sub 6sub/ -scFv fragments, thermore-sponsive copolymers, thermoprecipitation, affinity macroligands, bioseparation
in
Biotechnology and Bioengineering
volume
84
issue
4
pages
494 - 503
publisher
John Wiley & Sons
external identifiers
  • pmid:14574708
  • wos:000185945400013
  • scopus:0142104893
ISSN
1097-0290
DOI
10.1002/bit.10810
language
English
LU publication?
yes
id
bbda6901-2949-465a-9284-d1f0fbec1a72 (old id 124676)
date added to LUP
2007-07-02 12:50:59
date last changed
2018-09-16 03:41:04
@article{bbda6901-2949-465a-9284-d1f0fbec1a72,
  abstract     = {Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions. The present lack of widespread applications for this technique as compared to immobilized metal affinity chromatography (IMAC) may be related to the scarcity of well-characterized metal affinity macroligands (AML) and their applications to the number of different purification systems. In the present work we describe a detailed study of a new purification system using metal-loaded thermoresponsive copolymers as AML. The copolymers of vinylimidazole (VI) with N-isopropylacrylamide (NIPAM) were synthesized by radical polymerization with imidazole contents of 15 and 24 mol%. When loaded with Cu(II) and Ni(II) ions the copolymers selectively precipitated extracellularly expressed histidine-tagged single-chain Fv-antibody fragments (His6-scFv fragments) from the fermentation broth free from E. coli cells. Precipitation was induced by salt at mild temperatures and the bound antibody fragments were recovered by dissolving the protein-polymer complex in EDTA buffer and subsequent reprecipitation of the polymer. His6-scFv fragments were purified with yields of 91 and 80% and purification folds of 16 and 21 when Cu(II) and Ni(II) copolymers were used, respectively. The protein precipitation capacity of the Ni(II) copolymer showed a dependence on the VI concentration in the copolymer. The SDS-PAGE pattern showed significant purification of the antibody fragments. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 84: 494-503, 2003.},
  author       = {Kumar, Ashok and Wahlund, Per-Olof and Kepka, Cecilia and Galaev, Igor and Mattiasson, Bo},
  issn         = {1097-0290},
  keyword      = {metal chelate affinity precipitation,His/sub 6sub/ -scFv fragments,thermore-sponsive copolymers,thermoprecipitation,affinity macroligands,bioseparation},
  language     = {eng},
  number       = {4},
  pages        = {494--503},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers},
  url          = {http://dx.doi.org/10.1002/bit.10810},
  volume       = {84},
  year         = {2003},
}