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Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: Evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice

Shao, Ruijin; Nutu, Magdalena; Weijdegard, Birgitta; Egecioglu, Emil; Fernandez-Rodriguez, Julia; Tallet, Estelle; Goffin, Vincent; Ling, Charlotte LU and Billig, Hakan (2008) In Biology of Reproduction 79(4). p.748-757
Abstract
The anterior pituitary-derived hormone prolactin (PRL) signals through the PRL receptor (PRLR) and is important for female reproductive function in mammals. In contrast to the extensive studies of PRLR expression and regulation in human and mouse ovary and uterus, the mechanisms controlling the regulation of PRLR isoform expression in the fallopian tube are poorly understood. Because dynamic interaction of hormonal signaling in gonadal tissue and the pituitary or in gonadal tissues themselves in mammals suggests endocrine or paracrine regulation of PRLR expression, we questioned whether differential regulation of PRLR isoforms by PRL ovarian-derived estrogen (E-2) and progesterone (P-4) exists in the fallopian tube and pituitary of... (More)
The anterior pituitary-derived hormone prolactin (PRL) signals through the PRL receptor (PRLR) and is important for female reproductive function in mammals. In contrast to the extensive studies of PRLR expression and regulation in human and mouse ovary and uterus, the mechanisms controlling the regulation of PRLR isoform expression in the fallopian tube are poorly understood. Because dynamic interaction of hormonal signaling in gonadal tissue and the pituitary or in gonadal tissues themselves in mammals suggests endocrine or paracrine regulation of PRLR expression, we questioned whether differential regulation of PRLR isoforms by PRL ovarian-derived estrogen (E-2) and progesterone (P-4) exists in the fallopian tube and pituitary of prepubertal female mice. Western blot analysis showed distinct molecular separation of PRLR isoforms in mouse and human fallopian tubes, and cellular localization was found in mouse and human tubal epithelia but not in mouse tubal smooth muscle cells. These data support the concept of an isoform- and cell type-specific expression of PRLR in human and mouse fallopian tubes. Moreover, expression of the long form of PRLR decreased after PRL treatment and increased after blockage of endogenous PRL secretion by bromocriptine (an inhibitor of PRL secretion) in a time-dependent manner in mouse fallopian tube. The opposite regulation was observed in the pituitary. Treatment with exogenous E-2 or P-4 led to changes in PRLR expression in the fallopian tube Similar to those of PRL treatment. However, E-2 and P-4 did not affect PRLR expression in the pituitary. Estrogen had no effect on the long form of PRLR expression, whereas P-4 regulated the long form of PRLR in the fallopian tube, as did PRL. Taken together, the data from our comparative study provide evidence that PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. Furthermore, we found that ovarian steroid hormones selectively suppress the expression of PRLR isoforms in mouse fallopian tubes. These findings may contribute to our understanding of the mechanisms controlling PRLR isoform expression in the fallopian tube (in addition to ovary and uterus), with implications for female reproduction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ovarian steroid hormones, oviduct, pituitary, prolactin, prolactin receptor isoforms, prolactin receptor, estradiol, fallopian tube
in
Biology of Reproduction
volume
79
issue
4
pages
748 - 757
publisher
Soc Study Reproduction
external identifiers
  • wos:000259305300020
  • scopus:53349171480
ISSN
1529-7268
DOI
10.1095/biolreprod.108.070003
language
English
LU publication?
yes
id
7848c929-971e-4e62-8730-ce0d72f6b866 (old id 1246764)
date added to LUP
2008-11-19 11:03:47
date last changed
2017-01-01 05:12:18
@article{7848c929-971e-4e62-8730-ce0d72f6b866,
  abstract     = {The anterior pituitary-derived hormone prolactin (PRL) signals through the PRL receptor (PRLR) and is important for female reproductive function in mammals. In contrast to the extensive studies of PRLR expression and regulation in human and mouse ovary and uterus, the mechanisms controlling the regulation of PRLR isoform expression in the fallopian tube are poorly understood. Because dynamic interaction of hormonal signaling in gonadal tissue and the pituitary or in gonadal tissues themselves in mammals suggests endocrine or paracrine regulation of PRLR expression, we questioned whether differential regulation of PRLR isoforms by PRL ovarian-derived estrogen (E-2) and progesterone (P-4) exists in the fallopian tube and pituitary of prepubertal female mice. Western blot analysis showed distinct molecular separation of PRLR isoforms in mouse and human fallopian tubes, and cellular localization was found in mouse and human tubal epithelia but not in mouse tubal smooth muscle cells. These data support the concept of an isoform- and cell type-specific expression of PRLR in human and mouse fallopian tubes. Moreover, expression of the long form of PRLR decreased after PRL treatment and increased after blockage of endogenous PRL secretion by bromocriptine (an inhibitor of PRL secretion) in a time-dependent manner in mouse fallopian tube. The opposite regulation was observed in the pituitary. Treatment with exogenous E-2 or P-4 led to changes in PRLR expression in the fallopian tube Similar to those of PRL treatment. However, E-2 and P-4 did not affect PRLR expression in the pituitary. Estrogen had no effect on the long form of PRLR expression, whereas P-4 regulated the long form of PRLR in the fallopian tube, as did PRL. Taken together, the data from our comparative study provide evidence that PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. Furthermore, we found that ovarian steroid hormones selectively suppress the expression of PRLR isoforms in mouse fallopian tubes. These findings may contribute to our understanding of the mechanisms controlling PRLR isoform expression in the fallopian tube (in addition to ovary and uterus), with implications for female reproduction.},
  author       = {Shao, Ruijin and Nutu, Magdalena and Weijdegard, Birgitta and Egecioglu, Emil and Fernandez-Rodriguez, Julia and Tallet, Estelle and Goffin, Vincent and Ling, Charlotte and Billig, Hakan},
  issn         = {1529-7268},
  keyword      = {ovarian steroid hormones,oviduct,pituitary,prolactin,prolactin receptor isoforms,prolactin receptor,estradiol,fallopian tube},
  language     = {eng},
  number       = {4},
  pages        = {748--757},
  publisher    = {Soc Study Reproduction},
  series       = {Biology of Reproduction},
  title        = {Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: Evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice},
  url          = {http://dx.doi.org/10.1095/biolreprod.108.070003},
  volume       = {79},
  year         = {2008},
}