Advanced

Identification of new loci controlling collagen-induced arthritis in mouse using a partial advanced intercross and congenic strains

Popovic, M.; Ahlqvist, Emma LU ; Rockenbauer, Eszter LU ; Bockermann, Robert LU and Holmdahl, R. (2008) In Scandinavian Journal of Immunology 68(4). p.405-413
Abstract
Collagen-induced arthritis (CIA) is a well studied mouse model of the human disease rheumatoid arthritis (RA). Both CIA and RA are complex diseases affected by multiple genes as well as environmental factors. Identifying the genes that determine susceptibility to arthritis would give invaluable clues to the largely unknown aetiology of RA. In this study, we dissected a known locus, Cia6, as well as a genomic region on chromosome 14 with no previously known arthritis loci, using a partial advanced intercross and a collection of congenic strains. The chromosome 14 congenic fragment, containing the T-cell receptor alpha (Tcra) locus, was included based on the hypothesis that the Cia6 locus is caused by a polymorphism in the Tcr beta (Tcrb)... (More)
Collagen-induced arthritis (CIA) is a well studied mouse model of the human disease rheumatoid arthritis (RA). Both CIA and RA are complex diseases affected by multiple genes as well as environmental factors. Identifying the genes that determine susceptibility to arthritis would give invaluable clues to the largely unknown aetiology of RA. In this study, we dissected a known locus, Cia6, as well as a genomic region on chromosome 14 with no previously known arthritis loci, using a partial advanced intercross and a collection of congenic strains. The chromosome 14 congenic fragment, containing the T-cell receptor alpha (Tcra) locus, was included based on the hypothesis that the Cia6 locus is caused by a polymorphism in the Tcr beta (Tcrb) locus and that the two loci interact. Splitting up the congenic fragments revealed multiple loci affecting arthritis traits as well as production of collagen-specific autoantibodies. In total seven new loci were identified of which four were in the previously unlinked chromosome 14 region. Both Tcr loci were within CIA loci making them candidate susceptibility genes. The results demonstrate the importance of breaking up genetic regions in smaller fragments to identify the underlying complex set of loci. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Immunology
volume
68
issue
4
pages
405 - 413
publisher
Wiley-Blackwell
external identifiers
  • wos:000258978300007
  • scopus:51349130639
ISSN
1365-3083
DOI
10.1111/j.1365-3083.2008.02151.x
language
English
LU publication?
yes
id
069346b8-5bdb-4014-bbeb-3ac983edbc11 (old id 1247152)
date added to LUP
2008-11-20 09:26:13
date last changed
2017-01-01 06:25:49
@article{069346b8-5bdb-4014-bbeb-3ac983edbc11,
  abstract     = {Collagen-induced arthritis (CIA) is a well studied mouse model of the human disease rheumatoid arthritis (RA). Both CIA and RA are complex diseases affected by multiple genes as well as environmental factors. Identifying the genes that determine susceptibility to arthritis would give invaluable clues to the largely unknown aetiology of RA. In this study, we dissected a known locus, Cia6, as well as a genomic region on chromosome 14 with no previously known arthritis loci, using a partial advanced intercross and a collection of congenic strains. The chromosome 14 congenic fragment, containing the T-cell receptor alpha (Tcra) locus, was included based on the hypothesis that the Cia6 locus is caused by a polymorphism in the Tcr beta (Tcrb) locus and that the two loci interact. Splitting up the congenic fragments revealed multiple loci affecting arthritis traits as well as production of collagen-specific autoantibodies. In total seven new loci were identified of which four were in the previously unlinked chromosome 14 region. Both Tcr loci were within CIA loci making them candidate susceptibility genes. The results demonstrate the importance of breaking up genetic regions in smaller fragments to identify the underlying complex set of loci.},
  author       = {Popovic, M. and Ahlqvist, Emma and Rockenbauer, Eszter and Bockermann, Robert and Holmdahl, R.},
  issn         = {1365-3083},
  language     = {eng},
  number       = {4},
  pages        = {405--413},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Identification of new loci controlling collagen-induced arthritis in mouse using a partial advanced intercross and congenic strains},
  url          = {http://dx.doi.org/10.1111/j.1365-3083.2008.02151.x},
  volume       = {68},
  year         = {2008},
}