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Fusion of oil bodies in endosperm of oat grains

Heneen, Waheeb K.; Karlsson, Gunnel LU ; Brismar, Kerstin; Gummeson, Per-Olov; Marttila, Salla; Leonova, Svetlana; Carlsson, Anders S.; Bafor, Maureen; Banas, Antoni and Mattsson, Bengt, et al. (2008) In Planta 228(4). p.589-599
Abstract
Few microscopical studies have been made on lipid storage in oat grains, with variable results as to the extent of lipid accumulation in the starchy endosperm. Grains of medium- and high-lipid oat (Avena sativa L.) were studied at two developmental stages and at maturity, by light microscopy using different staining methods, and by scanning and transmission electron microscopy. Discrete oil bodies occurred in the aleurone layer, scutellum and embryo. In contrast, oil bodies in the starchy endosperm often had diffuse boundaries and fused with each other and with protein vacuoles during grain development, forming a continuous oil matrix between the protein and starch components. The different microscopical methods were confirmative to each... (More)
Few microscopical studies have been made on lipid storage in oat grains, with variable results as to the extent of lipid accumulation in the starchy endosperm. Grains of medium- and high-lipid oat (Avena sativa L.) were studied at two developmental stages and at maturity, by light microscopy using different staining methods, and by scanning and transmission electron microscopy. Discrete oil bodies occurred in the aleurone layer, scutellum and embryo. In contrast, oil bodies in the starchy endosperm often had diffuse boundaries and fused with each other and with protein vacuoles during grain development, forming a continuous oil matrix between the protein and starch components. The different microscopical methods were confirmative to each other regarding the coalescence of oil bodies, a phenomenon probably correlated with the reduced amount of oil-body associated proteins in the endosperm. This was supported experimentally by SDS-PAGE separation of oil-body proteins and immunoblotting and immunolocalization with antibodies against a 16 kD oil-body protein. Much more oil-body proteins per amount of oil occurred in the embryo and scutellum than in the endosperm. Immunolocalization of 14 and 16 kD oil-body associated proteins on sectioned grains resulted in more heavy labeling of the embryo, scutellum and aleurone layer than the rest of the endosperm. Observations on the appearance of oil bodies at an early stage of development pertain to the prevailing hypotheses of oil-body biogenesis. (Less)
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Contribution to journal
publication status
published
subject
keywords
oat, microscopy, fusion, aleurone, endosperm, oil body, oleosin
in
Planta
volume
228
issue
4
pages
589 - 599
publisher
Springer
external identifiers
  • wos:000259008800007
  • scopus:48949086188
ISSN
0032-0935
DOI
10.1007/s00425-008-0761-x
language
English
LU publication?
yes
id
85f06c75-064d-4f2a-938a-4d3c44804f8a (old id 1247159)
date added to LUP
2008-11-14 14:49:48
date last changed
2017-11-12 03:43:54
@article{85f06c75-064d-4f2a-938a-4d3c44804f8a,
  abstract     = {Few microscopical studies have been made on lipid storage in oat grains, with variable results as to the extent of lipid accumulation in the starchy endosperm. Grains of medium- and high-lipid oat (Avena sativa L.) were studied at two developmental stages and at maturity, by light microscopy using different staining methods, and by scanning and transmission electron microscopy. Discrete oil bodies occurred in the aleurone layer, scutellum and embryo. In contrast, oil bodies in the starchy endosperm often had diffuse boundaries and fused with each other and with protein vacuoles during grain development, forming a continuous oil matrix between the protein and starch components. The different microscopical methods were confirmative to each other regarding the coalescence of oil bodies, a phenomenon probably correlated with the reduced amount of oil-body associated proteins in the endosperm. This was supported experimentally by SDS-PAGE separation of oil-body proteins and immunoblotting and immunolocalization with antibodies against a 16 kD oil-body protein. Much more oil-body proteins per amount of oil occurred in the embryo and scutellum than in the endosperm. Immunolocalization of 14 and 16 kD oil-body associated proteins on sectioned grains resulted in more heavy labeling of the embryo, scutellum and aleurone layer than the rest of the endosperm. Observations on the appearance of oil bodies at an early stage of development pertain to the prevailing hypotheses of oil-body biogenesis.},
  author       = {Heneen, Waheeb K. and Karlsson, Gunnel and Brismar, Kerstin and Gummeson, Per-Olov and Marttila, Salla and Leonova, Svetlana and Carlsson, Anders S. and Bafor, Maureen and Banas, Antoni and Mattsson, Bengt and Debski, Henryk and Stymne, Sten},
  issn         = {0032-0935},
  keyword      = {oat,microscopy,fusion,aleurone,endosperm,oil body,oleosin},
  language     = {eng},
  number       = {4},
  pages        = {589--599},
  publisher    = {Springer},
  series       = {Planta},
  title        = {Fusion of oil bodies in endosperm of oat grains},
  url          = {http://dx.doi.org/10.1007/s00425-008-0761-x},
  volume       = {228},
  year         = {2008},
}