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Affinity partitioning for membrane purification exploiting the biotin-NeutrAvidin interaction - Model study of mixed liposomes and membranes

Barinaga-Rementeria Ramírez, Irene LU ; Mebrahtu, Sofia and Jergil, Bengt LU (2002) In Journal of Chromatography A 971(1-2). p.117-127
Abstract
Biotinylated negatively charged liposomes as well as membranes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using NeutrAvidin conjugated to dextran as affinity ligand. Both liposomes and membranes redistributed from top to bottom phase upon addition of NeutrAvidin-dextran. The presence of 35-60 mM Li2SO4 was necessary both to force the components into the top phase without ligand and for ligand-dependent redistribution into the bottom phase. Attaching biotin via a hexanamidohexanoyl spacer and an increased density of biotin or NeutrAvidin enhanced the affinity separation. The separation conditions in these model experiments provide a basis for affinity partitioning of membranes using other affinity... (More)
Biotinylated negatively charged liposomes as well as membranes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using NeutrAvidin conjugated to dextran as affinity ligand. Both liposomes and membranes redistributed from top to bottom phase upon addition of NeutrAvidin-dextran. The presence of 35-60 mM Li2SO4 was necessary both to force the components into the top phase without ligand and for ligand-dependent redistribution into the bottom phase. Attaching biotin via a hexanamidohexanoyl spacer and an increased density of biotin or NeutrAvidin enhanced the affinity separation. The separation conditions in these model experiments provide a basis for affinity partitioning of membranes using other affinity ligands. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Avidin, Liposomes, Biotin
in
Journal of Chromatography A
volume
971
issue
1-2
pages
117 - 127
publisher
Elsevier
external identifiers
  • pmid:12350107
  • wos:000178118300009
  • scopus:0037144173
ISSN
0021-9673
DOI
10.1016/S0021-9673(02)00841-5
language
English
LU publication?
yes
id
1566693c-af59-4720-af68-104b13aedd7d (old id 124769)
date added to LUP
2007-07-04 11:10:25
date last changed
2017-01-22 04:01:52
@article{1566693c-af59-4720-af68-104b13aedd7d,
  abstract     = {Biotinylated negatively charged liposomes as well as membranes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using NeutrAvidin conjugated to dextran as affinity ligand. Both liposomes and membranes redistributed from top to bottom phase upon addition of NeutrAvidin-dextran. The presence of 35-60 mM Li2SO4 was necessary both to force the components into the top phase without ligand and for ligand-dependent redistribution into the bottom phase. Attaching biotin via a hexanamidohexanoyl spacer and an increased density of biotin or NeutrAvidin enhanced the affinity separation. The separation conditions in these model experiments provide a basis for affinity partitioning of membranes using other affinity ligands.},
  author       = {Barinaga-Rementeria Ramírez, Irene and Mebrahtu, Sofia and Jergil, Bengt},
  issn         = {0021-9673},
  keyword      = {Avidin,Liposomes,Biotin},
  language     = {eng},
  number       = {1-2},
  pages        = {117--127},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Affinity partitioning for membrane purification exploiting the biotin-NeutrAvidin interaction - Model study of mixed liposomes and membranes},
  url          = {http://dx.doi.org/10.1016/S0021-9673(02)00841-5},
  volume       = {971},
  year         = {2002},
}