Affinity partitioning for membrane purification exploiting the biotin-NeutrAvidin interaction - Model study of mixed liposomes and membranes
(2002) In Journal of Chromatography A 971(1-2). p.117-127- Abstract
- Biotinylated negatively charged liposomes as well as membranes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using NeutrAvidin conjugated to dextran as affinity ligand. Both liposomes and membranes redistributed from top to bottom phase upon addition of NeutrAvidin-dextran. The presence of 35-60 mM Li2SO4 was necessary both to force the components into the top phase without ligand and for ligand-dependent redistribution into the bottom phase. Attaching biotin via a hexanamidohexanoyl spacer and an increased density of biotin or NeutrAvidin enhanced the affinity separation. The separation conditions in these model experiments provide a basis for affinity partitioning of membranes using other affinity... (More)
- Biotinylated negatively charged liposomes as well as membranes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using NeutrAvidin conjugated to dextran as affinity ligand. Both liposomes and membranes redistributed from top to bottom phase upon addition of NeutrAvidin-dextran. The presence of 35-60 mM Li2SO4 was necessary both to force the components into the top phase without ligand and for ligand-dependent redistribution into the bottom phase. Attaching biotin via a hexanamidohexanoyl spacer and an increased density of biotin or NeutrAvidin enhanced the affinity separation. The separation conditions in these model experiments provide a basis for affinity partitioning of membranes using other affinity ligands. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/124769
- author
- Barinaga-Rementeria Ramírez, Irene LU ; Mebrahtu, Sofia and Jergil, Bengt LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Avidin, Liposomes, Biotin
- in
- Journal of Chromatography A
- volume
- 971
- issue
- 1-2
- pages
- 117 - 127
- publisher
- Elsevier
- external identifiers
-
- pmid:12350107
- wos:000178118300009
- scopus:0037144173
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(02)00841-5
- language
- English
- LU publication?
- yes
- id
- 1566693c-af59-4720-af68-104b13aedd7d (old id 124769)
- date added to LUP
- 2016-04-01 15:47:39
- date last changed
- 2022-01-28 07:11:19
@article{1566693c-af59-4720-af68-104b13aedd7d,
abstract = {{Biotinylated negatively charged liposomes as well as membranes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using NeutrAvidin conjugated to dextran as affinity ligand. Both liposomes and membranes redistributed from top to bottom phase upon addition of NeutrAvidin-dextran. The presence of 35-60 mM Li2SO4 was necessary both to force the components into the top phase without ligand and for ligand-dependent redistribution into the bottom phase. Attaching biotin via a hexanamidohexanoyl spacer and an increased density of biotin or NeutrAvidin enhanced the affinity separation. The separation conditions in these model experiments provide a basis for affinity partitioning of membranes using other affinity ligands.}},
author = {{Barinaga-Rementeria Ramírez, Irene and Mebrahtu, Sofia and Jergil, Bengt}},
issn = {{0021-9673}},
keywords = {{Avidin; Liposomes; Biotin}},
language = {{eng}},
number = {{1-2}},
pages = {{117--127}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Affinity partitioning for membrane purification exploiting the biotin-NeutrAvidin interaction - Model study of mixed liposomes and membranes}},
url = {{http://dx.doi.org/10.1016/S0021-9673(02)00841-5}},
doi = {{10.1016/S0021-9673(02)00841-5}},
volume = {{971}},
year = {{2002}},
}