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Magneto-Optical Measurements of the Pigments in Fully Active Photosystem II Core Complexes from Plants

Smith, Paul J; Peterson Årsköld, Sindra LU ; Masters, Vanessa M; Wydrzynski, Tom; Styring, Stenbjörn LU ; Krausz, Elmars and Pace, Ron J (2002) In Biochemistry 41(6). p.1981-1989
Abstract
Preparation of a minimum PSII core complex from spinach is described, containing four Mn per reaction center (RC) and exhibiting high O2 evolving activity [~4000 mol of O2 (mg of chl)-1 h-1]. The complex consists of the CP47 and CP43 chlorophyll binding proteins, the RC D1/D2 pair, the cytochrome b559 subunits, and the Mn-stabilizing psbO (33 kDa) protein, all present in the same stoichiometric amounts found in the parent PSII membranes. Several small subunits are also present. The cyt b559 content is 1.0 per RC in core complexes and PSII membranes. The total chlorophyll content is 32 chl a and <1 chl b per RC, the lowest yet reported for any active PSII preparation. The core complex exhibits the characteristic EPR signals seen in the... (More)
Preparation of a minimum PSII core complex from spinach is described, containing four Mn per reaction center (RC) and exhibiting high O2 evolving activity [~4000 mol of O2 (mg of chl)-1 h-1]. The complex consists of the CP47 and CP43 chlorophyll binding proteins, the RC D1/D2 pair, the cytochrome b559 subunits, and the Mn-stabilizing psbO (33 kDa) protein, all present in the same stoichiometric amounts found in the parent PSII membranes. Several small subunits are also present. The cyt b559 content is 1.0 per RC in core complexes and PSII membranes. The total chlorophyll content is 32 chl a and <1 chl b per RC, the lowest yet reported for any active PSII preparation. The core complex exhibits the characteristic EPR signals seen in the S2 state of higher plant PSII. A procedure for preparing low-temperature samples of very high optical quality is developed, allowing detailed optical studies in the S1 and S2 states of the system to be made. Optical absorption, CD, and MCD spectra reveal unprecedented detail, including a prominent, well-resolved feature at 683.5 nm (14 630 cm-1) with a weaker partner at 187 cm-1 to higher energy. On the basis of band intensity, CD, and MCD arguments, these features are identified as the exciton split components of P680 in an intact, active reaction center special pair. Comparisons are made with solubilized D1/D2/cyt b559 material and cyanobacterial PSII. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
41
issue
6
pages
1981 - 1989
publisher
The American Chemical Society
external identifiers
  • wos:000173787400033
  • pmid:11827545
  • scopus:0037065695
ISSN
0006-2960
DOI
10.1021/bi0111202
language
English
LU publication?
yes
id
837eef74-18f6-492a-9800-a110c5263953 (old id 124890)
date added to LUP
2007-07-06 15:26:53
date last changed
2017-10-22 03:34:58
@article{837eef74-18f6-492a-9800-a110c5263953,
  abstract     = {Preparation of a minimum PSII core complex from spinach is described, containing four Mn per reaction center (RC) and exhibiting high O2 evolving activity [~4000 mol of O2 (mg of chl)-1 h-1]. The complex consists of the CP47 and CP43 chlorophyll binding proteins, the RC D1/D2 pair, the cytochrome b559 subunits, and the Mn-stabilizing psbO (33 kDa) protein, all present in the same stoichiometric amounts found in the parent PSII membranes. Several small subunits are also present. The cyt b559 content is 1.0 per RC in core complexes and PSII membranes. The total chlorophyll content is 32 chl a and &lt;1 chl b per RC, the lowest yet reported for any active PSII preparation. The core complex exhibits the characteristic EPR signals seen in the S2 state of higher plant PSII. A procedure for preparing low-temperature samples of very high optical quality is developed, allowing detailed optical studies in the S1 and S2 states of the system to be made. Optical absorption, CD, and MCD spectra reveal unprecedented detail, including a prominent, well-resolved feature at 683.5 nm (14 630 cm-1) with a weaker partner at 187 cm-1 to higher energy. On the basis of band intensity, CD, and MCD arguments, these features are identified as the exciton split components of P680 in an intact, active reaction center special pair. Comparisons are made with solubilized D1/D2/cyt b559 material and cyanobacterial PSII.},
  author       = {Smith, Paul J and Peterson Årsköld, Sindra and Masters, Vanessa M and Wydrzynski, Tom and Styring, Stenbjörn and Krausz, Elmars and Pace, Ron J},
  issn         = {0006-2960},
  language     = {eng},
  number       = {6},
  pages        = {1981--1989},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Magneto-Optical Measurements of the Pigments in Fully Active Photosystem II Core Complexes from Plants},
  url          = {http://dx.doi.org/10.1021/bi0111202},
  volume       = {41},
  year         = {2002},
}