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Detection of gastric Helicobacter species in free-ranging lynx (Lynx lynx) and red foxes (Vulpes vulpes) in Sweden

Morner, Torsten; Brojer, Caroline; Ryser-Degiorgis, Marie-Pierre; Gavier-Widen, Dolores; Nilsson, Hans-Olof LU and Wadström, Torkel LU (2008) In Journal of Wildlife Diseases 44(3). p.697-700
Abstract
Specimens of gastric mucosa and liver of 25 free-ranging Eurasian lynx (Lynx lynx), and four red foxes (Vulpes vulpes) shot in Sweden during 1999-2000, were investigated for the presence of Helicobacter species. Histopathology, bacteriologic culture and urease test, Helicobacter genus-specific 16S rDNA PCR analysis, and DNA sequence analysis were applied. Numerous Helicobacter-like organisms were observed histologically in the gastric mucosa of one fox. Helicobacter spp. were detected in the stomach by PCR analysis in 17 (68%) of the lynx and in three (755) of the foxes. Seven of the positive lynx were also positive in the urease test. PCR fragments, amplified from lynx and foxes, were sequenced and compared with those of known... (More)
Specimens of gastric mucosa and liver of 25 free-ranging Eurasian lynx (Lynx lynx), and four red foxes (Vulpes vulpes) shot in Sweden during 1999-2000, were investigated for the presence of Helicobacter species. Histopathology, bacteriologic culture and urease test, Helicobacter genus-specific 16S rDNA PCR analysis, and DNA sequence analysis were applied. Numerous Helicobacter-like organisms were observed histologically in the gastric mucosa of one fox. Helicobacter spp. were detected in the stomach by PCR analysis in 17 (68%) of the lynx and in three (755) of the foxes. Seven of the positive lynx were also positive in the urease test. PCR fragments, amplified from lynx and foxes, were sequenced and compared with those of known Helicobacter species. PCR products from lynx were closely related (>= 98% homology) to H. heilmannii, and PCR fragments from foxes demonstrated close homology to H. heilmannii and H. salomonis. No Helicobacter spp. or Helicobacter-like organisms could be cultured. The PCR analysis of the liver was negative for all animals, The pathologic significance of the presence of Helicobacter spp. in the stomach of free-ranging lynx and foxes remains uncertain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PCR, lynx, Helicobacter, bacteria, gastric mucosa, red fox, Vulpes
in
Journal of Wildlife Diseases
volume
44
issue
3
pages
697 - 700
publisher
Wildlife Disease Association
external identifiers
  • wos:000258668600018
  • scopus:52949144969
ISSN
0090-3558
language
English
LU publication?
yes
id
d5c28287-0a0b-4e04-9d56-fc26b08d8e19 (old id 1249321)
alternative location
http://www.jwildlifedis.org/cgi/content/abstract/44/3/697
date added to LUP
2008-11-10 13:57:12
date last changed
2017-01-01 06:10:31
@article{d5c28287-0a0b-4e04-9d56-fc26b08d8e19,
  abstract     = {Specimens of gastric mucosa and liver of 25 free-ranging Eurasian lynx (Lynx lynx), and four red foxes (Vulpes vulpes) shot in Sweden during 1999-2000, were investigated for the presence of Helicobacter species. Histopathology, bacteriologic culture and urease test, Helicobacter genus-specific 16S rDNA PCR analysis, and DNA sequence analysis were applied. Numerous Helicobacter-like organisms were observed histologically in the gastric mucosa of one fox. Helicobacter spp. were detected in the stomach by PCR analysis in 17 (68%) of the lynx and in three (755) of the foxes. Seven of the positive lynx were also positive in the urease test. PCR fragments, amplified from lynx and foxes, were sequenced and compared with those of known Helicobacter species. PCR products from lynx were closely related (>= 98% homology) to H. heilmannii, and PCR fragments from foxes demonstrated close homology to H. heilmannii and H. salomonis. No Helicobacter spp. or Helicobacter-like organisms could be cultured. The PCR analysis of the liver was negative for all animals, The pathologic significance of the presence of Helicobacter spp. in the stomach of free-ranging lynx and foxes remains uncertain.},
  author       = {Morner, Torsten and Brojer, Caroline and Ryser-Degiorgis, Marie-Pierre and Gavier-Widen, Dolores and Nilsson, Hans-Olof and Wadström, Torkel},
  issn         = {0090-3558},
  keyword      = {PCR,lynx,Helicobacter,bacteria,gastric mucosa,red fox,Vulpes},
  language     = {eng},
  number       = {3},
  pages        = {697--700},
  publisher    = {Wildlife Disease Association},
  series       = {Journal of Wildlife Diseases},
  title        = {Detection of gastric Helicobacter species in free-ranging lynx (Lynx lynx) and red foxes (Vulpes vulpes) in Sweden},
  volume       = {44},
  year         = {2008},
}