Basic characterization of two barley hemA promoter regionsreveals stem-loop structures and suggests a regulatory rolein Poaceae tetrapyrrole biosynthesis
(2001) In Plant Physiology and Biochemistry 39(2). p.155-160- Abstract
- 5-Aminolevulinic acid (ALA) is a common biosynthetic intermediate of both chlorophyll and heme. Glutamyl-tRNAGlu reductase, encoded by the hemA gene, is the initial enzyme of ALA biosynthesis. The presence of several hemA genes provides a possible explanation to the ability of plants to meet the different requirement of chlorophyll and heme using a common biosynthetic pathway. In this work, two barley (Hordeum vulgare L.) hemA genes were cloned from chromosomal DNA. The hemA1 and hemA2 genes are located at the long and short arm, respectively, of barley chromosome 5 (1H). Although having 98 % identical coding sequences the promoter regions of hemA1 and hemA2 are very different, which could explain their different expression patterns. The... (More)
- 5-Aminolevulinic acid (ALA) is a common biosynthetic intermediate of both chlorophyll and heme. Glutamyl-tRNAGlu reductase, encoded by the hemA gene, is the initial enzyme of ALA biosynthesis. The presence of several hemA genes provides a possible explanation to the ability of plants to meet the different requirement of chlorophyll and heme using a common biosynthetic pathway. In this work, two barley (Hordeum vulgare L.) hemA genes were cloned from chromosomal DNA. The hemA1 and hemA2 genes are located at the long and short arm, respectively, of barley chromosome 5 (1H). Although having 98 % identical coding sequences the promoter regions of hemA1 and hemA2 are very different, which could explain their different expression patterns. The first 170 nucleotides of the coding region of both genes contain several inverted repeat regions capable of forming stem-loop structures, which could be involved in the regulation of tetrapyrrole biosynthesis in species belonging to the Poaceae family. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/124997
- author
- Hansson, Mats LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5-aminolevulinate, chlorophyll, chromosomal location, glutamyl-tRNAGlu reductase, Hordeum vulgare, inverted repeat, regulation
- in
- Plant Physiology and Biochemistry
- volume
- 39
- issue
- 2
- pages
- 155 - 160
- publisher
- Elsevier
- external identifiers
-
- scopus:0035093957
- ISSN
- 1873-2690
- DOI
- 10.1016/S0981-9428(00)01227-4
- language
- English
- LU publication?
- yes
- id
- a2253680-c0ce-40ff-9238-58455f7e662a (old id 124997)
- date added to LUP
- 2016-04-01 17:03:40
- date last changed
- 2022-01-29 00:06:25
@article{a2253680-c0ce-40ff-9238-58455f7e662a,
abstract = {{5-Aminolevulinic acid (ALA) is a common biosynthetic intermediate of both chlorophyll and heme. Glutamyl-tRNAGlu reductase, encoded by the hemA gene, is the initial enzyme of ALA biosynthesis. The presence of several hemA genes provides a possible explanation to the ability of plants to meet the different requirement of chlorophyll and heme using a common biosynthetic pathway. In this work, two barley (Hordeum vulgare L.) hemA genes were cloned from chromosomal DNA. The hemA1 and hemA2 genes are located at the long and short arm, respectively, of barley chromosome 5 (1H). Although having 98 % identical coding sequences the promoter regions of hemA1 and hemA2 are very different, which could explain their different expression patterns. The first 170 nucleotides of the coding region of both genes contain several inverted repeat regions capable of forming stem-loop structures, which could be involved in the regulation of tetrapyrrole biosynthesis in species belonging to the Poaceae family.}},
author = {{Hansson, Mats}},
issn = {{1873-2690}},
keywords = {{5-aminolevulinate; chlorophyll; chromosomal location; glutamyl-tRNAGlu reductase; Hordeum vulgare; inverted repeat; regulation}},
language = {{eng}},
number = {{2}},
pages = {{155--160}},
publisher = {{Elsevier}},
series = {{Plant Physiology and Biochemistry}},
title = {{Basic characterization of two barley hemA promoter regionsreveals stem-loop structures and suggests a regulatory rolein Poaceae tetrapyrrole biosynthesis}},
url = {{http://dx.doi.org/10.1016/S0981-9428(00)01227-4}},
doi = {{10.1016/S0981-9428(00)01227-4}},
volume = {{39}},
year = {{2001}},
}