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On the Enzymatic Activation of NADH

Meijers, Rob; Morris, Richard J; Adolph, Hans W; Merli, Angelo; Lamzin, Victor S and Cedergren, Eila LU (2001) In Journal of Biological Chemistry 276(12). p.9316-9321
Abstract
Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
276
issue
12
pages
9316 - 9321
publisher
ASBMB
external identifiers
  • scopus:0035937753
ISSN
1083-351X
DOI
10.1074/jbc.M010870200
language
English
LU publication?
yes
id
69733d3b-43a8-41b9-96c2-b16ec8d829b4 (old id 125030)
date added to LUP
2007-07-06 11:50:51
date last changed
2018-10-03 10:33:49
@article{69733d3b-43a8-41b9-96c2-b16ec8d829b4,
  abstract     = {Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.},
  author       = {Meijers, Rob and Morris, Richard J and Adolph, Hans W and Merli, Angelo and Lamzin, Victor S and Cedergren, Eila},
  issn         = {1083-351X},
  language     = {eng},
  number       = {12},
  pages        = {9316--9321},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {On the Enzymatic Activation of NADH},
  url          = {http://dx.doi.org/10.1074/jbc.M010870200},
  volume       = {276},
  year         = {2001},
}