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Kinetics of the coupled reaction catalysed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase: Kinetics of a fusion protein of MDH and CS

Pettersson, Henrik LU ; Olsson, Peter LU ; Bülow, Leif LU and Pettersson, Gösta LU (2000) In European Journal of Biochemistry 267(16). p.5041-5046
Abstract
The mechanistic implications of the kinetic behaviour of a fusion protein of mitochondrial malate dehydrogenase and citrate synthase have been reanalysed in view of predictions based on experimentally determined kinetic parameter values for the dehydrogenase and synthase activities of the protein. The results show that the time-course of citrate formation from malate in the coupled reaction catalysed by the fusion protein can be most satisfactorily accounted for in terms of a free-diffusion mechanism when consideration is taken to the inhibitory effects of NADH and oxaloacetate on the malate dehydrogenase activity. The effect of aspartate aminotransferase on the coupled reaction is likewise fully consistent with that expected for a... (More)
The mechanistic implications of the kinetic behaviour of a fusion protein of mitochondrial malate dehydrogenase and citrate synthase have been reanalysed in view of predictions based on experimentally determined kinetic parameter values for the dehydrogenase and synthase activities of the protein. The results show that the time-course of citrate formation from malate in the coupled reaction catalysed by the fusion protein can be most satisfactorily accounted for in terms of a free-diffusion mechanism when consideration is taken to the inhibitory effects of NADH and oxaloacetate on the malate dehydrogenase activity. The effect of aspartate aminotransferase on the coupled reaction is likewise fully consistent with that expected for a free-diffusion mechanism. It is concluded that no tenable kinetic evidence is available to support the proposal that the fusion protein catalyses citrate formation from malate by a mechanism involving channelling of the intermediate oxaloacetate. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
fusion protein, channelling, malate dehydrogenase, Citrate (si)-Synthase, Oxaloacetic Acid, Recombinant Fusion Proteins, Saccharomyces cerevisiae, NAD, Models, Chemical, Mitochondria, Kinetics
in
European Journal of Biochemistry
volume
267
issue
16
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0033890234
ISSN
0014-2956
DOI
10.1046/j.1432-1327.2000.01558.x
language
English
LU publication?
yes
id
2f3ee9a2-75b9-4a85-9f17-ca0693890fef (old id 125154)
date added to LUP
2007-07-06 14:11:26
date last changed
2017-07-21 14:45:51
@article{2f3ee9a2-75b9-4a85-9f17-ca0693890fef,
  abstract     = {The mechanistic implications of the kinetic behaviour of a fusion protein of mitochondrial malate dehydrogenase and citrate synthase have been reanalysed in view of predictions based on experimentally determined kinetic parameter values for the dehydrogenase and synthase activities of the protein. The results show that the time-course of citrate formation from malate in the coupled reaction catalysed by the fusion protein can be most satisfactorily accounted for in terms of a free-diffusion mechanism when consideration is taken to the inhibitory effects of NADH and oxaloacetate on the malate dehydrogenase activity. The effect of aspartate aminotransferase on the coupled reaction is likewise fully consistent with that expected for a free-diffusion mechanism. It is concluded that no tenable kinetic evidence is available to support the proposal that the fusion protein catalyses citrate formation from malate by a mechanism involving channelling of the intermediate oxaloacetate.},
  author       = {Pettersson, Henrik and Olsson, Peter and Bülow, Leif and Pettersson, Gösta},
  issn         = {0014-2956},
  keyword      = {fusion protein,channelling,malate dehydrogenase,Citrate (si)-Synthase,Oxaloacetic Acid,Recombinant Fusion Proteins,Saccharomyces cerevisiae,NAD,Models, Chemical,Mitochondria,Kinetics},
  language     = {eng},
  number       = {16},
  pages        = {5041--5046},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Biochemistry},
  title        = {Kinetics of the coupled reaction catalysed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase: Kinetics of a fusion protein of MDH and CS},
  url          = {http://dx.doi.org/10.1046/j.1432-1327.2000.01558.x},
  volume       = {267},
  year         = {2000},
}