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Neutrophil degranulation mediates severe lung damage triggered by streptococcal M1 protein

Soehnlein, O.; Oehmcke, Sonja LU ; Ma, X.; Rothfuchs, A. G.; Frithiof, R.; van Rooijen, N.; Mörgelin, Matthias LU ; Herwald, Heiko LU and Lindbom, L. (2008) In European Respiratory Journal 32(2). p.405-412
Abstract
Streptococcus pyogenes of the M1 serotype can cause streptococcal toxic shock syndrome commonly associated with acute lung injury. The aim of the present study was to investigate the role of neutrophils and their secretion products in M1 protein-induced lung damage. The degranulation of neutrophills by M1 protein was studied in whole blood using marker analysis for individual granule subsets. In mice, M1 protein was injected intravenously and the lung damage was assessed by histology, electron microscopy, cell count in bronchoalveolar lavage fluid and analysis of lung vascular permeability. Comparisons were made in mice with intact white blood count, neutropenic mice and neutropenic mice injected with the secretion of activated... (More)
Streptococcus pyogenes of the M1 serotype can cause streptococcal toxic shock syndrome commonly associated with acute lung injury. The aim of the present study was to investigate the role of neutrophils and their secretion products in M1 protein-induced lung damage. The degranulation of neutrophills by M1 protein was studied in whole blood using marker analysis for individual granule subsets. In mice, M1 protein was injected intravenously and the lung damage was assessed by histology, electron microscopy, cell count in bronchoalveolar lavage fluid and analysis of lung vascular permeability. Comparisons were made in mice with intact white blood count, neutropenic mice and neutropenic mice injected with the secretion of activated neutrophils. In whole blood, M1 protein forms complexes with fibrinogen that bind to beta(2)-integrins on the neutrophil surface, resulting in degranulation of all four subsets of neutrophil granules. Intravenous injection of M1 protein into mice induced neutrophil accumulation in the lung, increase in vascular permeability and acute lung damage. Depletion of neutrophils from the circulation completely abrogated lung injury and vascular leakage. Interestingly, the lung damage was restored by injecting neutrophil secretion. The present data suggest that neutrophil granule proteins are directly responsible for lung damage induced by the streptococcal M1 protein. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
M1 protein, neutrophil granule proteins, lung injury, Streptococcus, pyogenes
in
European Respiratory Journal
volume
32
issue
2
pages
405 - 412
publisher
Eur Respiratory Soc
external identifiers
  • wos:000258417000023
  • scopus:55849125399
ISSN
1399-3003
DOI
10.1183/09031936.00173207
language
English
LU publication?
yes
id
b7b35379-2399-4508-8f83-a9448dbe0e25 (old id 1251929)
date added to LUP
2008-11-10 10:43:51
date last changed
2017-08-27 04:26:34
@article{b7b35379-2399-4508-8f83-a9448dbe0e25,
  abstract     = {Streptococcus pyogenes of the M1 serotype can cause streptococcal toxic shock syndrome commonly associated with acute lung injury. The aim of the present study was to investigate the role of neutrophils and their secretion products in M1 protein-induced lung damage. The degranulation of neutrophills by M1 protein was studied in whole blood using marker analysis for individual granule subsets. In mice, M1 protein was injected intravenously and the lung damage was assessed by histology, electron microscopy, cell count in bronchoalveolar lavage fluid and analysis of lung vascular permeability. Comparisons were made in mice with intact white blood count, neutropenic mice and neutropenic mice injected with the secretion of activated neutrophils. In whole blood, M1 protein forms complexes with fibrinogen that bind to beta(2)-integrins on the neutrophil surface, resulting in degranulation of all four subsets of neutrophil granules. Intravenous injection of M1 protein into mice induced neutrophil accumulation in the lung, increase in vascular permeability and acute lung damage. Depletion of neutrophils from the circulation completely abrogated lung injury and vascular leakage. Interestingly, the lung damage was restored by injecting neutrophil secretion. The present data suggest that neutrophil granule proteins are directly responsible for lung damage induced by the streptococcal M1 protein.},
  author       = {Soehnlein, O. and Oehmcke, Sonja and Ma, X. and Rothfuchs, A. G. and Frithiof, R. and van Rooijen, N. and Mörgelin, Matthias and Herwald, Heiko and Lindbom, L.},
  issn         = {1399-3003},
  keyword      = {M1 protein,neutrophil granule proteins,lung injury,Streptococcus,pyogenes},
  language     = {eng},
  number       = {2},
  pages        = {405--412},
  publisher    = {Eur Respiratory Soc},
  series       = {European Respiratory Journal},
  title        = {Neutrophil degranulation mediates severe lung damage triggered by streptococcal M1 protein},
  url          = {http://dx.doi.org/10.1183/09031936.00173207},
  volume       = {32},
  year         = {2008},
}