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Fractionation of cellulase and beta-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning

Brumbauer, Anikó; Johansson, Göte LU and Réczey, Kati (1999) In Bioseparation 7(6). p.287-295
Abstract
An aqueous two-phase system based on the two polymers poly(ethylene glycol) and dextran has been used for the fractionation of cellulase enzymes present in culture liquid obtained by fermentation with Trichoderma reesei. The activities of beta-glucosidase and glucanases were separated to high degree by using the two-phase systems for a counter-current distribution process in nine transfer steps. While the glucanases had high affinity to the poly(ethylene glycol) rich top phase the beta-glucosidase was enriched in the dextran-containing bottom phase. Multiple counter-current distribution performed indicates the heterogeneity of beta-glucosidase activities assuming at least four isoenzyme forms. One step concentration of beta-glucosidase by... (More)
An aqueous two-phase system based on the two polymers poly(ethylene glycol) and dextran has been used for the fractionation of cellulase enzymes present in culture liquid obtained by fermentation with Trichoderma reesei. The activities of beta-glucosidase and glucanases were separated to high degree by using the two-phase systems for a counter-current distribution process in nine transfer steps. While the glucanases had high affinity to the poly(ethylene glycol) rich top phase the beta-glucosidase was enriched in the dextran-containing bottom phase. Multiple counter-current distribution performed indicates the heterogeneity of beta-glucosidase activities assuming at least four isoenzyme forms. One step concentration of beta-glucosidase by using system with 46:1 phase volume ratio resulted in 16 times higher enzyme activity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aqueous two-phase systems, beta-glucosidase, cellulases, fractionation of enzymes, partitioning of proteins, Trichoderma reesei
in
Bioseparation
volume
7
issue
6
pages
287 - 295
publisher
Springer
external identifiers
  • scopus:0032859739
ISSN
1573-8272
DOI
10.1023/A:1008153418019
language
English
LU publication?
yes
id
c8388132-816b-4f12-9e3a-779bbf3b9642 (old id 125263)
date added to LUP
2007-07-04 14:18:19
date last changed
2017-01-01 07:09:12
@article{c8388132-816b-4f12-9e3a-779bbf3b9642,
  abstract     = {An aqueous two-phase system based on the two polymers poly(ethylene glycol) and dextran has been used for the fractionation of cellulase enzymes present in culture liquid obtained by fermentation with Trichoderma reesei. The activities of beta-glucosidase and glucanases were separated to high degree by using the two-phase systems for a counter-current distribution process in nine transfer steps. While the glucanases had high affinity to the poly(ethylene glycol) rich top phase the beta-glucosidase was enriched in the dextran-containing bottom phase. Multiple counter-current distribution performed indicates the heterogeneity of beta-glucosidase activities assuming at least four isoenzyme forms. One step concentration of beta-glucosidase by using system with 46:1 phase volume ratio resulted in 16 times higher enzyme activity.},
  author       = {Brumbauer, Anikó and Johansson, Göte and Réczey, Kati},
  issn         = {1573-8272},
  keyword      = {aqueous two-phase systems,beta-glucosidase,cellulases,fractionation of enzymes,partitioning of proteins,Trichoderma reesei},
  language     = {eng},
  number       = {6},
  pages        = {287--295},
  publisher    = {Springer},
  series       = {Bioseparation},
  title        = {Fractionation of cellulase and beta-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning},
  url          = {http://dx.doi.org/10.1023/A:1008153418019},
  volume       = {7},
  year         = {1999},
}