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Neutrophil secretion products pave the way for inflammatory monocytes

Soehnlein, Oliver; Zernecke, Alma; Eriksson, Einar E.; Rothfuchs, Antonio Gigliotti; Pham, Christine T.; Herwald, Heiko LU ; Bidzhekov, Kiril; Rottenberg, Martin E.; Weber, Christian and Lindbom, Lennart (2008) In Blood 112(4). p.1461-1471
Abstract
The leukocyte response in inflammation is characterized by an initial recruitment of polymorphonuclear leukocytes (PMN) preceding a second wave of monocytes to the site of injury or infection. In the mouse, 2 populations of monocytes have been identified, Gr1-CCR2-CX3CR1(hi) resident monocytes and Gr1+CCR2+CX3CR1(lo) inflammatory monocytes. Here, intravital microscopy of the musculus cremasterand a subcutaneous air pouch model were used to investigate a possible link between PMN extravasation and the subsequent emigration of inflammatory monocytes in response to local stimulation with PAR In mice that were made neutropenic by injection of a PMN-depleting antibody, the extravasation of inflammatory monocytes, but not resident monocytes, was... (More)
The leukocyte response in inflammation is characterized by an initial recruitment of polymorphonuclear leukocytes (PMN) preceding a second wave of monocytes to the site of injury or infection. In the mouse, 2 populations of monocytes have been identified, Gr1-CCR2-CX3CR1(hi) resident monocytes and Gr1+CCR2+CX3CR1(lo) inflammatory monocytes. Here, intravital microscopy of the musculus cremasterand a subcutaneous air pouch model were used to investigate a possible link between PMN extravasation and the subsequent emigration of inflammatory monocytes in response to local stimulation with PAR In mice that were made neutropenic by injection of a PMN-depleting antibody, the extravasation of inflammatory monocytes, but not resident monocytes, was markedly reduced compared with mice with intact white blood cell count but was restored by local treatment with secretion of activated PMN. Components of the PMN secretion were found to and further examination revealed PMN-derived LL-37 and heparin-binding protein (HBP/CAP37/azurocidin) as primary mediators of the recruitment of inflammatory monocytes via activation of formyl-peptide receptors. These data show that LL-37 and HBP specifically stimulate mobilization of inflammatory monocytes. This cellular cross-talk functionally results in enhanced cytokine levels and increased bacterial clearance, thus boosting the early immune response. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
112
issue
4
pages
1461 - 1471
publisher
American Society of Hematology
external identifiers
  • wos:000258392300073
  • scopus:51649104412
ISSN
1528-0020
DOI
10.1182/blood-2008-02-139634
language
English
LU publication?
yes
id
6065c890-34dd-4d21-90bb-ce816e26cc62 (old id 1252921)
date added to LUP
2008-10-31 09:04:06
date last changed
2017-11-12 03:20:11
@article{6065c890-34dd-4d21-90bb-ce816e26cc62,
  abstract     = {The leukocyte response in inflammation is characterized by an initial recruitment of polymorphonuclear leukocytes (PMN) preceding a second wave of monocytes to the site of injury or infection. In the mouse, 2 populations of monocytes have been identified, Gr1-CCR2-CX3CR1(hi) resident monocytes and Gr1+CCR2+CX3CR1(lo) inflammatory monocytes. Here, intravital microscopy of the musculus cremasterand a subcutaneous air pouch model were used to investigate a possible link between PMN extravasation and the subsequent emigration of inflammatory monocytes in response to local stimulation with PAR In mice that were made neutropenic by injection of a PMN-depleting antibody, the extravasation of inflammatory monocytes, but not resident monocytes, was markedly reduced compared with mice with intact white blood cell count but was restored by local treatment with secretion of activated PMN. Components of the PMN secretion were found to and further examination revealed PMN-derived LL-37 and heparin-binding protein (HBP/CAP37/azurocidin) as primary mediators of the recruitment of inflammatory monocytes via activation of formyl-peptide receptors. These data show that LL-37 and HBP specifically stimulate mobilization of inflammatory monocytes. This cellular cross-talk functionally results in enhanced cytokine levels and increased bacterial clearance, thus boosting the early immune response.},
  author       = {Soehnlein, Oliver and Zernecke, Alma and Eriksson, Einar E. and Rothfuchs, Antonio Gigliotti and Pham, Christine T. and Herwald, Heiko and Bidzhekov, Kiril and Rottenberg, Martin E. and Weber, Christian and Lindbom, Lennart},
  issn         = {1528-0020},
  language     = {eng},
  number       = {4},
  pages        = {1461--1471},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {Neutrophil secretion products pave the way for inflammatory monocytes},
  url          = {http://dx.doi.org/10.1182/blood-2008-02-139634},
  volume       = {112},
  year         = {2008},
}