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Investigation into A antigen expression on O-2 heterozygous group O-labeled red blood cell units

Yazer, Mark H.; Hult, Annika LU ; Hellberg, Åsa LU ; Hosseini Maaf, Bahram LU ; Palcic, Monica M. and Olsson, Martin L LU (2008) In Transfusion 48(8). p.1650-1657
Abstract
BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O-2, encodes full-length protein with Gly268Arg. While reports vary, O-2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O-2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O-2 alleles. The... (More)
BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O-2, encodes full-length protein with Gly268Arg. While reports vary, O-2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O-2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O-2 alleles. The following tests were performed on randomly selected O-2 samples (number): adsorption-elution with anti-A (3), flow cytometry (15), plasma enzyme activity (4), and attempts to convert group O RBCs to A (2) with O-2 plasma and titration of plasma anti-A/-A(1) (3). RESULTS: Forty O-2-heterozygous donors were identified (5.1%). Adsorption-elution and sensitive flow cytometry did not reveal A antigens on O-2 RBCs. Plasma enzyme analysis failed to show GTA activity above baseline; O-2 plasma was unable to add measurable A antigens to O RBCs. Titers of anti-A/-A(1) appeared reduced in O-2 plasma but did not cause ABO typing discrepancies. No immediate hemolysis or adverse reactions were reported following transfusion of O-2 RBCs to six evaluable group O recipients. CONCLUSIONS: Other than lower plasma anti-A titers, GTA activity was not found in these O-2 samples. Neither automated blood grouping discrepancies nor clinical problems related to transfusing these O-2 units were observed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transfusion
volume
48
issue
8
pages
1650 - 1657
publisher
Wiley-Blackwell
external identifiers
  • wos:000258077800019
  • scopus:48249146230
ISSN
1537-2995
DOI
10.1111/j.1537-2995.2008.01732.x
language
English
LU publication?
yes
id
22c9c15f-66a5-4711-be39-3bad246d197b (old id 1253566)
date added to LUP
2008-10-29 08:24:54
date last changed
2017-01-01 06:30:25
@article{22c9c15f-66a5-4711-be39-3bad246d197b,
  abstract     = {BACKGROUND: There are two principal types of group O alleles; deletional alleles feature 261delG leading to nonfunctional truncated protein. Nondeletional alleles have the consensus guanosine at residue 261. The major nondeletional allele, O-2, encodes full-length protein with Gly268Arg. While reports vary, O-2 has been proposed to encode weakly functional A-glycosyltransferase (GTA). The main objective of this study was to evaluate if GTA activity is detectable in O-2 donors. STUDY DESIGN AND METHODS: Donor samples from Pittsburgh and Lund were ABO typed by automated methods. DNA was extracted from 779 group O donors whose red blood cells (RBCs) were available for transfusion. ABO genotyping identified those with O-2 alleles. The following tests were performed on randomly selected O-2 samples (number): adsorption-elution with anti-A (3), flow cytometry (15), plasma enzyme activity (4), and attempts to convert group O RBCs to A (2) with O-2 plasma and titration of plasma anti-A/-A(1) (3). RESULTS: Forty O-2-heterozygous donors were identified (5.1%). Adsorption-elution and sensitive flow cytometry did not reveal A antigens on O-2 RBCs. Plasma enzyme analysis failed to show GTA activity above baseline; O-2 plasma was unable to add measurable A antigens to O RBCs. Titers of anti-A/-A(1) appeared reduced in O-2 plasma but did not cause ABO typing discrepancies. No immediate hemolysis or adverse reactions were reported following transfusion of O-2 RBCs to six evaluable group O recipients. CONCLUSIONS: Other than lower plasma anti-A titers, GTA activity was not found in these O-2 samples. Neither automated blood grouping discrepancies nor clinical problems related to transfusing these O-2 units were observed.},
  author       = {Yazer, Mark H. and Hult, Annika and Hellberg, Åsa and Hosseini Maaf, Bahram and Palcic, Monica M. and Olsson, Martin L},
  issn         = {1537-2995},
  language     = {eng},
  number       = {8},
  pages        = {1650--1657},
  publisher    = {Wiley-Blackwell},
  series       = {Transfusion},
  title        = {Investigation into A antigen expression on O-2 heterozygous group O-labeled red blood cell units},
  url          = {http://dx.doi.org/10.1111/j.1537-2995.2008.01732.x},
  volume       = {48},
  year         = {2008},
}