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Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase

Pettersson, Henrik LU and Pettersson, Gösta LU (1999) In European Journal of Biochemistry 262(2). p.371-376
Abstract
Transient-state kinetic experiments and analyses have been performed to examine the validity of hitherto unchallenged evidence proposed to be indicative of a channelled transfer of triose phosphates from aldolase to glyceraldehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behaviour of the examined aldolase-dehydrogenase reactions is fully consistent with a free-diffusion mechanism of metabolite transfer.
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aldolase, dehydrogenase, channelling, glycolysis
in
European Journal of Biochemistry
volume
262
issue
2
pages
371 - 376
publisher
Wiley-Blackwell
external identifiers
  • scopus:0033152812
ISSN
0014-2956
DOI
10.1046/j.1432-1327.1999.00386.x
language
English
LU publication?
yes
id
7f1aea37-256d-42bf-8197-83c76c71fa04 (old id 125396)
date added to LUP
2016-04-01 15:54:04
date last changed
2022-01-28 07:55:02
@article{7f1aea37-256d-42bf-8197-83c76c71fa04,
  abstract     = {{Transient-state kinetic experiments and analyses have been performed to examine the validity of hitherto unchallenged evidence proposed to be indicative of a channelled transfer of triose phosphates from aldolase to glyceraldehyde-3-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. The results lend no support to such proposals, but show that the kinetic behaviour of the examined aldolase-dehydrogenase reactions is fully consistent with a free-diffusion mechanism of metabolite transfer.}},
  author       = {{Pettersson, Henrik and Pettersson, Gösta}},
  issn         = {{0014-2956}},
  keywords     = {{aldolase; dehydrogenase; channelling; glycolysis}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{371--376}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{European Journal of Biochemistry}},
  title        = {{Mechanism of metabolite transfer in coupled two-enzyme reactions involving aldolase}},
  url          = {{http://dx.doi.org/10.1046/j.1432-1327.1999.00386.x}},
  doi          = {{10.1046/j.1432-1327.1999.00386.x}},
  volume       = {{262}},
  year         = {{1999}},
}