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Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit

Willows, R D; Hansson, Mats LU ; Beale, S I; Laurberg, Martin LU and Al-Karadaghi, Salam LU (1999) In Acta Crystallographica. Section D: Biological Crystallography D55. p.689-690
Abstract
The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bacteriochlorophyll., chlorophyll, magnesium chelatase BchI subunit
in
Acta Crystallographica. Section D: Biological Crystallography
volume
D55
pages
689 - 690
publisher
International Union of Crystallography
external identifiers
  • scopus:13044280254
ISSN
1399-0047
DOI
10.1107/S0907444998014759
language
English
LU publication?
yes
id
f974d91d-7759-44b6-a1a9-1a4b3b33e368 (old id 125452)
date added to LUP
2007-07-09 09:22:35
date last changed
2017-08-06 04:39:56
@article{f974d91d-7759-44b6-a1a9-1a4b3b33e368,
  abstract     = {The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.},
  author       = {Willows, R D and Hansson, Mats and Beale, S I and Laurberg, Martin and Al-Karadaghi, Salam},
  issn         = {1399-0047},
  keyword      = {bacteriochlorophyll.,chlorophyll,magnesium chelatase BchI subunit},
  language     = {eng},
  pages        = {689--690},
  publisher    = {International Union of Crystallography},
  series       = {Acta Crystallographica. Section D: Biological Crystallography},
  title        = {Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit},
  url          = {http://dx.doi.org/10.1107/S0907444998014759},
  volume       = {D55},
  year         = {1999},
}