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Asthmatic symptoms among pupils in relation to microbial dust exposure in schools in Taiyuan, China

Zhao, Zhuohui; Sebastian, Aleksandra LU ; Larsson, Lennart LU ; Wang, Zhuanhua; Zhang, Zheng and Norback, Dan (2008) In Pediatric Allergy and Immunology 19(5). p.455-465
Abstract
Microbial exposure has been indicated as significant in the development of asthma and allergy among children. The aim of the study was to test whether microbial exposure and allergens in the school environment are associated with asthmatic symptoms in pupils. Data on asthmatic symptoms and respiratory infections were collected through a questionnaire survey among 1993 pupils aged 11-15 yr in 10 randomly selected schools in Taiyuan, China. Settled dust in classrooms was analysed using tandem gas chromatography-mass spectrometry for 3-hydroxy fatty acids, marker of lipopolysaccharide (LPS) from endotoxin, muramic acid (MuA), marker of bacteria and ergosterol (Erg) for fungi, quantifying both culturable and non-culturable microbes. A total of... (More)
Microbial exposure has been indicated as significant in the development of asthma and allergy among children. The aim of the study was to test whether microbial exposure and allergens in the school environment are associated with asthmatic symptoms in pupils. Data on asthmatic symptoms and respiratory infections were collected through a questionnaire survey among 1993 pupils aged 11-15 yr in 10 randomly selected schools in Taiyuan, China. Settled dust in classrooms was analysed using tandem gas chromatography-mass spectrometry for 3-hydroxy fatty acids, marker of lipopolysaccharide (LPS) from endotoxin, muramic acid (MuA), marker of bacteria and ergosterol (Erg) for fungi, quantifying both culturable and non-culturable microbes. A total of 29.8% reported daytime attacks of breathlessness, 8.4% wheeze and 1.2% had doctor's diagnosed asthma. Generally, MuA was negatively associated with wheeze and daytime attacks of breathlessness, the latter of which was negatively associated with Erg to a weaker extent. Total concentration of LPS was positively associated with daytime attacks of breathlessness, but shorter lengths of LPS, C10, C12 and C14 LPS were negatively associated with either wheezing or daytime attacks of breathlessness. For MuA and C10 and C12 of LPS, the associations were independent of airborne allergens and classroom crowdedness, and even independent of the other two microbial markers for MuA. Microbial exposure indicated by certain chemical markers (e.g. MuA) could be protective for asthmatic symptoms, but for LPS (endotoxin), the picture is more complex, varying by different lengths of fatty acids of LPS. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
moulds, indoor environment, endotoxin, dust, bacteria, allergy, asthma
in
Pediatric Allergy and Immunology
volume
19
issue
5
pages
455 - 465
publisher
Wiley-Blackwell
external identifiers
  • wos:000257570900012
  • scopus:47349095974
ISSN
0905-6157
DOI
10.1111/j.1399-3038.2007.00664.x
language
English
LU publication?
yes
id
3fc847b6-d6a6-4ff8-a919-cc12977604cc (old id 1254620)
date added to LUP
2008-11-03 14:06:32
date last changed
2017-10-01 04:25:17
@article{3fc847b6-d6a6-4ff8-a919-cc12977604cc,
  abstract     = {Microbial exposure has been indicated as significant in the development of asthma and allergy among children. The aim of the study was to test whether microbial exposure and allergens in the school environment are associated with asthmatic symptoms in pupils. Data on asthmatic symptoms and respiratory infections were collected through a questionnaire survey among 1993 pupils aged 11-15 yr in 10 randomly selected schools in Taiyuan, China. Settled dust in classrooms was analysed using tandem gas chromatography-mass spectrometry for 3-hydroxy fatty acids, marker of lipopolysaccharide (LPS) from endotoxin, muramic acid (MuA), marker of bacteria and ergosterol (Erg) for fungi, quantifying both culturable and non-culturable microbes. A total of 29.8% reported daytime attacks of breathlessness, 8.4% wheeze and 1.2% had doctor's diagnosed asthma. Generally, MuA was negatively associated with wheeze and daytime attacks of breathlessness, the latter of which was negatively associated with Erg to a weaker extent. Total concentration of LPS was positively associated with daytime attacks of breathlessness, but shorter lengths of LPS, C10, C12 and C14 LPS were negatively associated with either wheezing or daytime attacks of breathlessness. For MuA and C10 and C12 of LPS, the associations were independent of airborne allergens and classroom crowdedness, and even independent of the other two microbial markers for MuA. Microbial exposure indicated by certain chemical markers (e.g. MuA) could be protective for asthmatic symptoms, but for LPS (endotoxin), the picture is more complex, varying by different lengths of fatty acids of LPS.},
  author       = {Zhao, Zhuohui and Sebastian, Aleksandra and Larsson, Lennart and Wang, Zhuanhua and Zhang, Zheng and Norback, Dan},
  issn         = {0905-6157},
  keyword      = {moulds,indoor environment,endotoxin,dust,bacteria,allergy,asthma},
  language     = {eng},
  number       = {5},
  pages        = {455--465},
  publisher    = {Wiley-Blackwell},
  series       = {Pediatric Allergy and Immunology},
  title        = {Asthmatic symptoms among pupils in relation to microbial dust exposure in schools in Taiyuan, China},
  url          = {http://dx.doi.org/10.1111/j.1399-3038.2007.00664.x},
  volume       = {19},
  year         = {2008},
}