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Simultaneous detection of spin-coupled and decoupled QA- EPR-signals in Photosystem II complexes isolated with isoelectric focusing

Geijer, Paulina LU ; Peterson Årsköld, Sindra LU ; Härndal, Ulrika; Styring, Stenbjörn LU and Sundby, Cecilia (1998) In Photosynthesis Research 58(3). p.231-243
Abstract
The Photosystem II multisubunit protein complex can be extracted from thylakoid membranes with non-ionic detergents and subjected to various spectroscopical and biochemical investigations. This paper shows that after extraction with dodecyl-beta-D-maltoside, several Photosystem II complexes could be resolved by isoelectric focusing. Structurally, the various Photosystem II complexes differed from each other in polypeptide composition, especially with regard to the chlorophyll a/b-binding proteins, which gave rise to differing isoelectric points. Functionally, the various Photosystem II complexes differed from each other on the acceptor side, as judged by acceptor side-dependent electron transfer and electron paramagnetic resonance (EPR).... (More)
The Photosystem II multisubunit protein complex can be extracted from thylakoid membranes with non-ionic detergents and subjected to various spectroscopical and biochemical investigations. This paper shows that after extraction with dodecyl-beta-D-maltoside, several Photosystem II complexes could be resolved by isoelectric focusing. Structurally, the various Photosystem II complexes differed from each other in polypeptide composition, especially with regard to the chlorophyll a/b-binding proteins, which gave rise to differing isoelectric points. Functionally, the various Photosystem II complexes differed from each other on the acceptor side, as judged by acceptor side-dependent electron transfer and electron paramagnetic resonance (EPR). The Q_{A}^- Fe2+-signal (g = 1.84), arising from Q_{A}^- spin-coupled to the acceptor-side iron, and a radical signal arising from decoupled Q_{A}^- (g = 2.0045) could be detected simultaneously in some of the Photosystem II complexes, and the amount of each of the two signals were inversely related. The results are discussed in relation to previously known heterogeneities in Photosystem II. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
EPR, IEF, polypeptide composition, PS II core, PS II heterogeneity, Q_{A}
in
Photosynthesis Research
volume
58
issue
3
pages
231 - 243
publisher
Springer
external identifiers
  • scopus:0032434528
ISSN
0166-8595
DOI
10.1023/A:1006194800627
language
English
LU publication?
yes
id
697654ed-4f2c-4120-a199-3614852afec7 (old id 125511)
date added to LUP
2007-07-04 16:57:09
date last changed
2017-01-01 06:55:12
@article{697654ed-4f2c-4120-a199-3614852afec7,
  abstract     = {The Photosystem II multisubunit protein complex can be extracted from thylakoid membranes with non-ionic detergents and subjected to various spectroscopical and biochemical investigations. This paper shows that after extraction with dodecyl-beta-D-maltoside, several Photosystem II complexes could be resolved by isoelectric focusing. Structurally, the various Photosystem II complexes differed from each other in polypeptide composition, especially with regard to the chlorophyll a/b-binding proteins, which gave rise to differing isoelectric points. Functionally, the various Photosystem II complexes differed from each other on the acceptor side, as judged by acceptor side-dependent electron transfer and electron paramagnetic resonance (EPR). The Q_{A}^- Fe2+-signal (g = 1.84), arising from Q_{A}^- spin-coupled to the acceptor-side iron, and a radical signal arising from decoupled Q_{A}^- (g = 2.0045) could be detected simultaneously in some of the Photosystem II complexes, and the amount of each of the two signals were inversely related. The results are discussed in relation to previously known heterogeneities in Photosystem II.},
  author       = {Geijer, Paulina and Peterson Årsköld, Sindra and Härndal, Ulrika and Styring, Stenbjörn and Sundby, Cecilia},
  issn         = {0166-8595},
  keyword      = {EPR,IEF,polypeptide composition,PS II core,PS II heterogeneity,Q_{A}},
  language     = {eng},
  number       = {3},
  pages        = {231--243},
  publisher    = {Springer},
  series       = {Photosynthesis Research},
  title        = {Simultaneous detection of spin-coupled and decoupled QA- EPR-signals in Photosystem II complexes isolated with isoelectric focusing},
  url          = {http://dx.doi.org/10.1023/A:1006194800627},
  volume       = {58},
  year         = {1998},
}