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Effect of the head-group geometry of amino acid-based cationic surfactants on interaction with plasmid DNA

Jadhav, Vaibhav; Maiti, Souvik; Dasgupta, Antara; Das, Prasanta Kumar; Dias, Rita LU ; Miguel, Maria LU and Lindman, Björn LU (2008) In Biomacromolecules 9(7). p.1852-1859
Abstract
The interaction between DNA and different types of amino acid-based cationic surfactants was investigated. Particular attention was directed to determine the extent of influence of surfactant head-group geometry toward tuning the interaction behavior of these surfactants with DNA. An overview is obtained by gel retardation assay, isothermal titration calorimetry, fluorescence spectroscopy, and circular dichroism at different mole ratios of surfactant/DNA; also, cell viability was assessed. The studies show that the surfactants with more complex/ bulkier hydrophobic head group interact more strongly with DNA but exclude ethidium bromide less efficiently; thus, the accessibility of DNA to small molecules is preserved to a certain extent. The... (More)
The interaction between DNA and different types of amino acid-based cationic surfactants was investigated. Particular attention was directed to determine the extent of influence of surfactant head-group geometry toward tuning the interaction behavior of these surfactants with DNA. An overview is obtained by gel retardation assay, isothermal titration calorimetry, fluorescence spectroscopy, and circular dichroism at different mole ratios of surfactant/DNA; also, cell viability was assessed. The studies show that the surfactants with more complex/ bulkier hydrophobic head group interact more strongly with DNA but exclude ethidium bromide less efficiently; thus, the accessibility of DNA to small molecules is preserved to a certain extent. The presence of more hydrophobic groups surrounding the positive amino charge also gave rise to a significantly lower cytotoxicity. The surfactant self-assembly pattern is quite different without and with DNA, illustrating the roles of electrostatic and steric effects in determining the effective shape of a surfactant molecule. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
9
issue
7
pages
1852 - 1859
publisher
The American Chemical Society
external identifiers
  • wos:000257639500019
  • scopus:48449097336
ISSN
1526-4602
DOI
10.1021/bm8000765
language
English
LU publication?
yes
id
cf7331db-de6f-43a1-a82e-4dd2b44af9e0 (old id 1255149)
date added to LUP
2008-10-17 13:45:52
date last changed
2017-08-27 03:59:34
@article{cf7331db-de6f-43a1-a82e-4dd2b44af9e0,
  abstract     = {The interaction between DNA and different types of amino acid-based cationic surfactants was investigated. Particular attention was directed to determine the extent of influence of surfactant head-group geometry toward tuning the interaction behavior of these surfactants with DNA. An overview is obtained by gel retardation assay, isothermal titration calorimetry, fluorescence spectroscopy, and circular dichroism at different mole ratios of surfactant/DNA; also, cell viability was assessed. The studies show that the surfactants with more complex/ bulkier hydrophobic head group interact more strongly with DNA but exclude ethidium bromide less efficiently; thus, the accessibility of DNA to small molecules is preserved to a certain extent. The presence of more hydrophobic groups surrounding the positive amino charge also gave rise to a significantly lower cytotoxicity. The surfactant self-assembly pattern is quite different without and with DNA, illustrating the roles of electrostatic and steric effects in determining the effective shape of a surfactant molecule.},
  author       = {Jadhav, Vaibhav and Maiti, Souvik and Dasgupta, Antara and Das, Prasanta Kumar and Dias, Rita and Miguel, Maria and Lindman, Björn},
  issn         = {1526-4602},
  language     = {eng},
  number       = {7},
  pages        = {1852--1859},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Effect of the head-group geometry of amino acid-based cationic surfactants on interaction with plasmid DNA},
  url          = {http://dx.doi.org/10.1021/bm8000765},
  volume       = {9},
  year         = {2008},
}