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In-field determination of nanomolar nitrite in seawater using a sequential injection technique combined with solid phase enrichment and colorimetric detection

Chen, Guohe; Yuan, Dongxing; Huang, Yongming; Zhang, Min and Bergman, Maria LU (2008) In Analytica Chimica Acta 620(1-2). p.82-88
Abstract
A novel sequential injection method for the determination of nitrite at nanomolar level in seawater samples has been developed. The pink azo compound was formed based on the Griess reaction and quantitatively adsorbed onto a Sep-Pak C18 cartridge. The enriched azo compound was rinsed with water and ethanol (28%, v/v) in turn, and then eluted with an eluent containing 26.6% (v/v) ethanol and 0.108mol L-1 H2SO4. Finally the azo compound was measured using a spectrophotometer at 543 nm. Under the optimized conditions, the linear calibration ranges were 0.71-42.9 nmol L-1 for a 150-mL sample and 35.7-429 nmol L-1 for a 15-mL sample. The relative standard deviation of 8 measurements was 1.44% for 14.3 nmol L-1 nitrite. For the 150 mL sample,... (More)
A novel sequential injection method for the determination of nitrite at nanomolar level in seawater samples has been developed. The pink azo compound was formed based on the Griess reaction and quantitatively adsorbed onto a Sep-Pak C18 cartridge. The enriched azo compound was rinsed with water and ethanol (28%, v/v) in turn, and then eluted with an eluent containing 26.6% (v/v) ethanol and 0.108mol L-1 H2SO4. Finally the azo compound was measured using a spectrophotometer at 543 nm. Under the optimized conditions, the linear calibration ranges were 0.71-42.9 nmol L-1 for a 150-mL sample and 35.7-429 nmol L-1 for a 15-mL sample. The relative standard deviation of 8 measurements was 1.44% for 14.3 nmol L-1 nitrite. For the 150 mL sample, the detection limit was estimated to be 0.1 nmol L-1. The throughput of the method was about 4 samples per hour. The proposed method has been successfully applied to the in-field determination of nanomolar concentrations of nitrite in seawater. (c) 2008 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sequential injection, nitrite, seawater, solid phase extraction
in
Analytica Chimica Acta
volume
620
issue
1-2
pages
82 - 88
publisher
Elsevier
external identifiers
  • wos:000257553200010
  • scopus:46549083047
ISSN
1873-4324
DOI
10.1016/j.aca.2008.05.019
language
English
LU publication?
yes
id
edd88f77-2d0b-4064-aff4-ceec2cb891b5 (old id 1255198)
date added to LUP
2008-10-16 13:33:22
date last changed
2017-08-13 04:11:53
@article{edd88f77-2d0b-4064-aff4-ceec2cb891b5,
  abstract     = {A novel sequential injection method for the determination of nitrite at nanomolar level in seawater samples has been developed. The pink azo compound was formed based on the Griess reaction and quantitatively adsorbed onto a Sep-Pak C18 cartridge. The enriched azo compound was rinsed with water and ethanol (28%, v/v) in turn, and then eluted with an eluent containing 26.6% (v/v) ethanol and 0.108mol L-1 H2SO4. Finally the azo compound was measured using a spectrophotometer at 543 nm. Under the optimized conditions, the linear calibration ranges were 0.71-42.9 nmol L-1 for a 150-mL sample and 35.7-429 nmol L-1 for a 15-mL sample. The relative standard deviation of 8 measurements was 1.44% for 14.3 nmol L-1 nitrite. For the 150 mL sample, the detection limit was estimated to be 0.1 nmol L-1. The throughput of the method was about 4 samples per hour. The proposed method has been successfully applied to the in-field determination of nanomolar concentrations of nitrite in seawater. (c) 2008 Elsevier B.V. All rights reserved.},
  author       = {Chen, Guohe and Yuan, Dongxing and Huang, Yongming and Zhang, Min and Bergman, Maria},
  issn         = {1873-4324},
  keyword      = {sequential injection,nitrite,seawater,solid phase extraction},
  language     = {eng},
  number       = {1-2},
  pages        = {82--88},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {In-field determination of nanomolar nitrite in seawater using a sequential injection technique combined with solid phase enrichment and colorimetric detection},
  url          = {http://dx.doi.org/10.1016/j.aca.2008.05.019},
  volume       = {620},
  year         = {2008},
}