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Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus

van de Werken, Harmen; Verhaart, Marcel; VanFossen, Amy; Willquist, Karin LU ; Lewis, Derrick; Nichols, Jason; Goorissen, Heleen; Mongodin, Emmanuel; Nelson, Karen and van Niel, Ed LU , et al. (2008) In Applied and Environmental Microbiology 74(21). p.6720-6729
Abstract
Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments

cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2, and hydrogen. Its broad substrate

range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an

attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus,

consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described.

Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity

for cellulose, hemicellulose, pectin, and starch,... (More)
Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments

cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2, and hydrogen. Its broad substrate

range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an

attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus,

consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described.

Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity

for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric

and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate

pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic

pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose,

were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced

ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome

analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar

transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars

unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range

of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocelluloseutilizing,

biofuel-producing bacterium. (Less)
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keywords
genome annotation extreme thermophile hydrogen production Caldicellulosiruptor saccharolyticus
in
Applied and Environmental Microbiology
volume
74
issue
21
pages
6720 - 6729
publisher
American Society for Microbiology
external identifiers
  • wos:000260429600029
  • scopus:55049112099
ISSN
0099-2240
DOI
10.1128/AEM.00968-08
language
English
LU publication?
yes
id
43c23eb6-a95e-4db7-b04b-1a31fcdd0186 (old id 1260514)
date added to LUP
2008-11-03 12:54:57
date last changed
2017-11-05 03:46:59
@article{43c23eb6-a95e-4db7-b04b-1a31fcdd0186,
  abstract     = {Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments<br/><br>
cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2, and hydrogen. Its broad substrate<br/><br>
range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an<br/><br>
attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus,<br/><br>
consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described.<br/><br>
Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity<br/><br>
for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric<br/><br>
and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate<br/><br>
pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic<br/><br>
pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose,<br/><br>
were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced<br/><br>
ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome<br/><br>
analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar<br/><br>
transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars<br/><br>
unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range<br/><br>
of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocelluloseutilizing,<br/><br>
biofuel-producing bacterium.},
  author       = {van de Werken, Harmen and Verhaart, Marcel and VanFossen, Amy and Willquist, Karin and Lewis, Derrick and Nichols, Jason and Goorissen, Heleen and Mongodin, Emmanuel and Nelson, Karen and van Niel, Ed and Stams, Alfons and Ward, Donald and de Vos, Willem and van der Oost, John and Kelly, Rober and Kengen, Servé},
  issn         = {0099-2240},
  keyword      = {genome annotation extreme thermophile hydrogen production Caldicellulosiruptor saccharolyticus},
  language     = {eng},
  number       = {21},
  pages        = {6720--6729},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus},
  url          = {http://dx.doi.org/10.1128/AEM.00968-08},
  volume       = {74},
  year         = {2008},
}