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Occurrence of Apoptosis, Secondary Necrosis and Cytolysis in Eosinophilic Nasal Polyps.

Uller, Lena LU ; Andersson, Morgan LU ; Greiff, Lennart LU ; Persson, Carl LU and Erjefält, Jonas LU (2004) In American Journal of Respiratory and Critical Care Medicine 170(7). p.742-747
Abstract
The paradigm states that inflammatory cells disappear from airway tissues through apoptosis and phagocytosis. However, cells may also be cleared through primary cytolysis, necrosis secondary to apoptosis, or transepithelial migration. This study examines the occurrence of apoptosis, secondary necrosis, and cytolysis of eosinophils in human nasal polyps in vivo and blood eosinophils in vitro. Eosinophils abounded in subepithelium and in paracellular epithelial pathways. Macrophages commonly occurred but without engulfed eosinophils. Scattered cells, including epithelial cells, were stained by antibody to the caspase cleavage product of poly(ADP-ribose) polymerase. Few cells were apoptotic (stained by terminal deoxy RNase nick end labeling).... (More)
The paradigm states that inflammatory cells disappear from airway tissues through apoptosis and phagocytosis. However, cells may also be cleared through primary cytolysis, necrosis secondary to apoptosis, or transepithelial migration. This study examines the occurrence of apoptosis, secondary necrosis, and cytolysis of eosinophils in human nasal polyps in vivo and blood eosinophils in vitro. Eosinophils abounded in subepithelium and in paracellular epithelial pathways. Macrophages commonly occurred but without engulfed eosinophils. Scattered cells, including epithelial cells, were stained by antibody to the caspase cleavage product of poly(ADP-ribose) polymerase. Few cells were apoptotic (stained by terminal deoxy RNase nick end labeling). Of more than 3,000 examined tissue eosinophils, 110 were caspase cleavage positive, but only one was apoptotic. Transmission electron microscopy analysis of more than 500 eosinophils revealed viable and cytolytic eosinophils but not apoptosis, secondary necrosis, or engulfment of eosinophils. Plasma cells but neither epithelial cells nor eosinophils exhibited apoptotic ultrastructural morphology. Eosinophils in vitro exhibited different stages of apoptosis, ending with secondary necrosis distinct from in vivo eosinophil cytolysis. Our results show that the clearance of eosinophils from nasal polyps largely occurs through nonapoptosis pathways, including cytolysis and paraepithelial migration, and they challenge the belief that apoptosis is important for clearance of eosinophils from respiratory tissues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
terminal, poly(ADP-ribose) polymerase, electron microscopy, epithelium, deoxy RNase nick end labeling
in
American Journal of Respiratory and Critical Care Medicine
volume
170
issue
7
pages
742 - 747
publisher
Am Thoracic Soc
external identifiers
  • pmid:15229095
  • wos:000224111800007
  • scopus:4644289075
ISSN
1535-4970
DOI
10.1164/rccm.200402-240OC
language
English
LU publication?
yes
id
c11333ef-b67d-473c-99c5-b42c8b863c91 (old id 126070)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15229095&dopt=Abstract
date added to LUP
2007-07-20 07:57:17
date last changed
2017-12-10 03:50:39
@article{c11333ef-b67d-473c-99c5-b42c8b863c91,
  abstract     = {The paradigm states that inflammatory cells disappear from airway tissues through apoptosis and phagocytosis. However, cells may also be cleared through primary cytolysis, necrosis secondary to apoptosis, or transepithelial migration. This study examines the occurrence of apoptosis, secondary necrosis, and cytolysis of eosinophils in human nasal polyps in vivo and blood eosinophils in vitro. Eosinophils abounded in subepithelium and in paracellular epithelial pathways. Macrophages commonly occurred but without engulfed eosinophils. Scattered cells, including epithelial cells, were stained by antibody to the caspase cleavage product of poly(ADP-ribose) polymerase. Few cells were apoptotic (stained by terminal deoxy RNase nick end labeling). Of more than 3,000 examined tissue eosinophils, 110 were caspase cleavage positive, but only one was apoptotic. Transmission electron microscopy analysis of more than 500 eosinophils revealed viable and cytolytic eosinophils but not apoptosis, secondary necrosis, or engulfment of eosinophils. Plasma cells but neither epithelial cells nor eosinophils exhibited apoptotic ultrastructural morphology. Eosinophils in vitro exhibited different stages of apoptosis, ending with secondary necrosis distinct from in vivo eosinophil cytolysis. Our results show that the clearance of eosinophils from nasal polyps largely occurs through nonapoptosis pathways, including cytolysis and paraepithelial migration, and they challenge the belief that apoptosis is important for clearance of eosinophils from respiratory tissues.},
  author       = {Uller, Lena and Andersson, Morgan and Greiff, Lennart and Persson, Carl and Erjefält, Jonas},
  issn         = {1535-4970},
  keyword      = {terminal,poly(ADP-ribose) polymerase,electron microscopy,epithelium,deoxy RNase nick end labeling},
  language     = {eng},
  number       = {7},
  pages        = {742--747},
  publisher    = {Am Thoracic Soc},
  series       = {American Journal of Respiratory and Critical Care Medicine},
  title        = {Occurrence of Apoptosis, Secondary Necrosis and Cytolysis in Eosinophilic Nasal Polyps.},
  url          = {http://dx.doi.org/10.1164/rccm.200402-240OC},
  volume       = {170},
  year         = {2004},
}