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Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.

Khayyami, M; Pérez Pita, M T; Peña Garcia, N; Johansson, Gillis LU ; Danielsson, Bengt LU and Larsson, Per-Olof LU (1998) In Talanta 45(3). p.557-563
Abstract
A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acetylthiocholine, Acetylcholine esterase, Biosensor, RVC-electrode, Amperometric, Thiocholine, Mediator, Superporous agarose gel
in
Talanta
volume
45
issue
3
pages
557 - 563
publisher
Elsevier
external identifiers
  • pmid:18967037
  • scopus:0031941469
ISSN
1873-3573
DOI
10.1016/S0039-9140(97)00182-3
language
English
LU publication?
yes
id
bccb2ac7-6838-4dfb-927c-c570fb7a254e (old id 1261779)
date added to LUP
2008-12-01 11:35:06
date last changed
2017-09-10 04:35:58
@article{bccb2ac7-6838-4dfb-927c-c570fb7a254e,
  abstract     = {A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.},
  author       = {Khayyami, M and Pérez Pita, M T and Peña Garcia, N and Johansson, Gillis and Danielsson, Bengt and Larsson, Per-Olof},
  issn         = {1873-3573},
  keyword      = {Acetylthiocholine,Acetylcholine esterase,Biosensor,RVC-electrode,Amperometric,Thiocholine,Mediator,Superporous agarose gel},
  language     = {eng},
  number       = {3},
  pages        = {557--563},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.},
  url          = {http://dx.doi.org/10.1016/S0039-9140(97)00182-3},
  volume       = {45},
  year         = {1998},
}