Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.

Khayyami, M ; Pérez Pita, M T ; Peña Garcia, N ; Johansson, Gillis LU ; Danielsson, Bengt LU and Larsson, Per-Olof LU (1998) In Talanta 45(3). p.557-563
Abstract
A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acetylthiocholine, Acetylcholine esterase, Biosensor, RVC-electrode, Amperometric, Thiocholine, Mediator, Superporous agarose gel
in
Talanta
volume
45
issue
3
pages
557 - 563
publisher
Elsevier
external identifiers
  • pmid:18967037
  • scopus:0031941469
ISSN
1873-3573
DOI
10.1016/S0039-9140(97)00182-3
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Analytical Chemistry (S/LTH) (011001004)
id
bccb2ac7-6838-4dfb-927c-c570fb7a254e (old id 1261779)
date added to LUP
2016-04-01 16:39:23
date last changed
2022-01-28 21:12:15
@article{bccb2ac7-6838-4dfb-927c-c570fb7a254e,
  abstract     = {{A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.}},
  author       = {{Khayyami, M and Pérez Pita, M T and Peña Garcia, N and Johansson, Gillis and Danielsson, Bengt and Larsson, Per-Olof}},
  issn         = {{1873-3573}},
  keywords     = {{Acetylthiocholine; Acetylcholine esterase; Biosensor; RVC-electrode; Amperometric; Thiocholine; Mediator; Superporous agarose gel}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{557--563}},
  publisher    = {{Elsevier}},
  series       = {{Talanta}},
  title        = {{Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material.}},
  url          = {{http://dx.doi.org/10.1016/S0039-9140(97)00182-3}},
  doi          = {{10.1016/S0039-9140(97)00182-3}},
  volume       = {{45}},
  year         = {{1998}},
}