Advanced

Costimulation blockade in transplantation of nerve allografts: long-term effects.

Kvist, Martin LU ; Kanje, Martin LU ; Ekberg, Henrik LU ; Corbascio, Matthias and Dahlin, Lars B (2008) In Journal of the peripheral nervous system : JPNS 13(3). p.200-207
Abstract
Costimulation blockade can prevent rejection of nerve allografts in short-term studies. We tested if costimulation blockade also prevented rejection of nerve allografts in long-term experiments, thereby improving functional recovery. A 7-mm sciatic nerve defect in C57/BL6 mice was bridged either by nerve allografts from Balb/C mice or by isogenic nerve grafts (isografts) from C57/BL6 mice. Costimulation blockade in the form of a triple treatment with anti-LFA-1, anti-CD40L, and CTLA4Ig was given at post-operative days 0, 2, 4, and 6 (intraperitoneal). Control mice (placebo; allografts) with nerve grafts were treated with isotype antibodies during the same time period. After 49 days, tetanic muscle force, wet weight of gastrocnemius muscle,... (More)
Costimulation blockade can prevent rejection of nerve allografts in short-term studies. We tested if costimulation blockade also prevented rejection of nerve allografts in long-term experiments, thereby improving functional recovery. A 7-mm sciatic nerve defect in C57/BL6 mice was bridged either by nerve allografts from Balb/C mice or by isogenic nerve grafts (isografts) from C57/BL6 mice. Costimulation blockade in the form of a triple treatment with anti-LFA-1, anti-CD40L, and CTLA4Ig was given at post-operative days 0, 2, 4, and 6 (intraperitoneal). Control mice (placebo; allografts) with nerve grafts were treated with isotype antibodies during the same time period. After 49 days, tetanic muscle force, wet weight of gastrocnemius muscle, histology, and morphometry in the tibial nerve were evaluated. Costimulation blockade diminished rejection of the nerve allografts. Axons bridged the graft. Treatment increased wet weight of the gastrocnemius muscle and resulted in a higher mean myelin area/nerve fiber in the tibial nerve distal to the nerve grafts. Tetanic muscle force and number of axons in tibial nerve showed no differences between groups. We conclude that rejection is suppressed by costimulation blockade. Treatment improves recovery of target muscle and myelination after nerve allografting. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the peripheral nervous system : JPNS
volume
13
issue
3
pages
200 - 207
publisher
John Wiley & Sons
external identifiers
  • wos:000259613000002
  • pmid:18844786
  • scopus:52949088299
ISSN
1529-8027
DOI
10.1111/j.1529-8027.2008.00178.x
language
English
LU publication?
yes
id
032e99ee-b105-4099-9304-2f8b4b89fef2 (old id 1262389)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18844786?dopt=Abstract
date added to LUP
2008-11-10 16:29:55
date last changed
2017-01-01 05:30:24
@article{032e99ee-b105-4099-9304-2f8b4b89fef2,
  abstract     = {Costimulation blockade can prevent rejection of nerve allografts in short-term studies. We tested if costimulation blockade also prevented rejection of nerve allografts in long-term experiments, thereby improving functional recovery. A 7-mm sciatic nerve defect in C57/BL6 mice was bridged either by nerve allografts from Balb/C mice or by isogenic nerve grafts (isografts) from C57/BL6 mice. Costimulation blockade in the form of a triple treatment with anti-LFA-1, anti-CD40L, and CTLA4Ig was given at post-operative days 0, 2, 4, and 6 (intraperitoneal). Control mice (placebo; allografts) with nerve grafts were treated with isotype antibodies during the same time period. After 49 days, tetanic muscle force, wet weight of gastrocnemius muscle, histology, and morphometry in the tibial nerve were evaluated. Costimulation blockade diminished rejection of the nerve allografts. Axons bridged the graft. Treatment increased wet weight of the gastrocnemius muscle and resulted in a higher mean myelin area/nerve fiber in the tibial nerve distal to the nerve grafts. Tetanic muscle force and number of axons in tibial nerve showed no differences between groups. We conclude that rejection is suppressed by costimulation blockade. Treatment improves recovery of target muscle and myelination after nerve allografting.},
  author       = {Kvist, Martin and Kanje, Martin and Ekberg, Henrik and Corbascio, Matthias and Dahlin, Lars B},
  issn         = {1529-8027},
  language     = {eng},
  number       = {3},
  pages        = {200--207},
  publisher    = {John Wiley & Sons},
  series       = {Journal of the peripheral nervous system : JPNS},
  title        = {Costimulation blockade in transplantation of nerve allografts: long-term effects.},
  url          = {http://dx.doi.org/10.1111/j.1529-8027.2008.00178.x},
  volume       = {13},
  year         = {2008},
}